Concurrent inhibition by chlorpromazine of concanavalin A-induced lymphocyte aggregation and mitogenesis

A PLANT lectin from extract of Jack bean (concanavalin A) is known to initiate transformation in vitro of T lymphocytes into large, proliferating blast-like cells 1 . Knowledge of the mechanism whereby con A induces such changes is incomplete, but a necessary first step seems to involve the binding...

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Bibliographic Details
Published in:Nature (London) 1975-08, Vol.256 (5520), p.744-745
Main Authors: FERGUSON, RONALD M, SCHMIDTKE, JON R, SIMMONS, RICHARD L
Format: Article
Language:English
Subjects:
Calcium - pharmacology
Cell Aggregation - drug effects
Cell Membrane - drug effects
Chlorpromazine - pharmacology
Chlorpromazine - toxicity
Concanavalin A - antagonists & inhibitors
Cyclic AMP - pharmacology
Cyclic GMP - pharmacology
DNA Replication - drug effects
Dose-Response Relationship, Drug
Humanities and Social Sciences
letter
Lymphocyte Activation - drug effects
Lymphocytes - drug effects
Lymphocytes - ultrastructure
Mitosis - drug effects
multidisciplinary
Science
Science (multidisciplinary)
Time Factors
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Summary:A PLANT lectin from extract of Jack bean (concanavalin A) is known to initiate transformation in vitro of T lymphocytes into large, proliferating blast-like cells 1 . Knowledge of the mechanism whereby con A induces such changes is incomplete, but a necessary first step seems to involve the binding of con A to receptors on the surface membrane of the lymphocyte. Within minutes of binding of a mitogen, recognisable alterations in lymphocyte surface membrane physiology have been noted. These include increased phospholipid incorporation into the membrane 2 , increased fluxes of ionic potassium 3 and calcium 4 , and increased uptake of nucleosides 5 , sugar 6 , and amino acids 7 . In addition, con A has been reported to increase the fluidity of lymphocyte membranes after binding 8 . Internalisation of con A is not required for lymphocyte activation so that con A binding to the lymphocyte surface membrane of T cells seems to be sufficient to induce blastogenesis 9 .
ISSN:0028-0836
1476-4687
DOI:10.1038/256744a0