Cyclic AMP-mediated transformation of rat cells transformed by temperature-sensitive mouse sarcoma virus

SEVERAL lines of evidence suggest that adenosine 3′,5′-cyclic monophosphate (cyclic AMP) is involved in the regulation of growth and other properties of cultured cells (for review, see ref. 1). Specifically, various reports have shown a correlation between the abnormal growth and morphological prope...

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Bibliographic Details
Published in:Nature (London) 1975-09, Vol.257 (5521), p.58-60
Main Authors: SOMERS, KENNETH D, RACHMELER, MARTIN, CHRISTENSEN, MARY
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cell Transformation, Neoplastic - drug effects
Cyclic AMP - analogs & derivatives
Cyclic AMP - metabolism
Cyclic AMP - pharmacology
Drug Synergism
Gammaretrovirus
Humanities and Social Sciences
letter
multidisciplinary
Mutation
Rats
Sarcoma Viruses, Murine
Science
Structure-Activity Relationship
Temperature
Theophylline - pharmacology
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Summary:SEVERAL lines of evidence suggest that adenosine 3′,5′-cyclic monophosphate (cyclic AMP) is involved in the regulation of growth and other properties of cultured cells (for review, see ref. 1). Specifically, various reports have shown a correlation between the abnormal growth and morphological properties of virus-transformed cells and low levels of cyclic AMP 2,3 . Treatment of these transformed cells with cyclic AMP, cyclic AMP analogues, or agents that raise cyclic AMP levels restores growth and morphological properties characteristic of normal cells 4–7 . The use of cells transformed by temperature-sensitive (ts) mutants of Rous sarcoma virus (RSV) or mouse sarcoma virus (MSV) 8,9 as well as ts host cell mutants 10,11 strengthened the evidence relating viral transformation and cyclic AMP levels. Other evidence obtained in similar virally transformed cell systems, however, has failed to confirm the inverse relationship between cyclic AMP levels and cell growth rate and transformation by RNA and DNA tumour viruses 12–15 . We now report that the exogenous addition of cyclic AMP mediates the morphological transformation of rat cells transformed by a ts mutant of MSV at the non-permissive temperature. Correspondingly, expression of the transformed phenotype by cells grown at the permissive temperature is associated with increased levels of endogenous cyclic AMP.
ISSN:0028-0836
1476-4687
DOI:10.1038/257058a0