Procarcinogen activation and hormonal control of cell proliferation in differentiated primary adult rat liver cell cultures

CONCERTED interactions among hormones and nutrients control animal cell proliferation 1 . For example, in the rat, insulin, glucagon, glucocorticoid, parathyroid hormone, iodothyronines and amino acids regulate normal hepatic proliferation—a prominent system for studying epithelial cell growth contr...

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Bibliographic Details
Published in:Nature (London) 1977-05, Vol.267 (5606), p.58-61
Main Authors: LEFFERT, H. L., MORAN, T., BOORSTEIN, R., KOCH, K. S.
Format: Article
Language:English
Subjects:
2-Acetylaminofluorene - metabolism
Albumins - physiology
Animals
Arginine - pharmacology
Cell Division - drug effects
Cells, Cultured - metabolism
Cells, Cultured - physiology
Fluorenes - metabolism
Humanities and Social Sciences
Hydrocortisone - pharmacology
Inosine - pharmacology
Insulin - pharmacology
letter
Liver - cytology
Liver - metabolism
Liver - physiology
multidisciplinary
Ornithine - metabolism
Rats
Science
Science (multidisciplinary)
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Summary:CONCERTED interactions among hormones and nutrients control animal cell proliferation 1 . For example, in the rat, insulin, glucagon, glucocorticoid, parathyroid hormone, iodothyronines and amino acids regulate normal hepatic proliferation—a prominent system for studying epithelial cell growth control 2 . Endocrine and nutritional factors also modify the efficiency with which chemicals produce experimental tumours in these animals notably, the induction of hepatomas by aromatic amines 8 . We report here observations with a novel primary monolayer adult rat liver culture system which may prove helpful towards studying these interactions under defined conditions 4 . These cells proliferate in response to hormones and nutrients; they maintain differentiated hepatic functions such as albumin and arginine biosynthesis for at least 8–10 days after plating; and they metabolise the procarcinogen N -2-acetylaminofluorene (AAF) into a proximate form, N -hydroxy-AAF (ref. 5).
ISSN:0028-0836
1476-4687
DOI:10.1038/267058a0