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Procarcinogen activation and hormonal control of cell proliferation in differentiated primary adult rat liver cell cultures
CONCERTED interactions among hormones and nutrients control animal cell proliferation 1 . For example, in the rat, insulin, glucagon, glucocorticoid, parathyroid hormone, iodothyronines and amino acids regulate normal hepatic proliferation—a prominent system for studying epithelial cell growth contr...
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| Published in: | Nature (London) 1977-05, Vol.267 (5606), p.58-61 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c2260-35136a5347988dfe4615d751fc7aa0848b3e213432754f474c6c5dedcf5e98d53 |
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| cites | cdi_FETCH-LOGICAL-c2260-35136a5347988dfe4615d751fc7aa0848b3e213432754f474c6c5dedcf5e98d53 |
| container_end_page | 61 |
| container_issue | 5606 |
| container_start_page | 58 |
| container_title | Nature (London) |
| container_volume | 267 |
| creator | LEFFERT, H. L. MORAN, T. BOORSTEIN, R. KOCH, K. S. |
| description | CONCERTED interactions among hormones and nutrients control animal cell proliferation
1
. For example, in the rat, insulin, glucagon, glucocorticoid, parathyroid hormone, iodothyronines and amino acids regulate normal hepatic proliferation—a prominent system for studying epithelial cell growth control
2
. Endocrine and nutritional factors also modify the efficiency with which chemicals produce experimental tumours in these animals notably, the induction of hepatomas by aromatic amines
8
. We report here observations with a novel primary monolayer adult rat liver culture system which may prove helpful towards studying these interactions under defined conditions
4
. These cells proliferate in response to hormones and nutrients; they maintain differentiated hepatic functions such as albumin and arginine biosynthesis for at least 8–10 days after plating; and they metabolise the procarcinogen
N
-2-acetylaminofluorene (AAF) into a proximate form,
N
-hydroxy-AAF (ref. 5). |
| doi_str_mv | 10.1038/267058a0 |
| format | article |
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1
. For example, in the rat, insulin, glucagon, glucocorticoid, parathyroid hormone, iodothyronines and amino acids regulate normal hepatic proliferation—a prominent system for studying epithelial cell growth control
2
. Endocrine and nutritional factors also modify the efficiency with which chemicals produce experimental tumours in these animals notably, the induction of hepatomas by aromatic amines
8
. We report here observations with a novel primary monolayer adult rat liver culture system which may prove helpful towards studying these interactions under defined conditions
4
. These cells proliferate in response to hormones and nutrients; they maintain differentiated hepatic functions such as albumin and arginine biosynthesis for at least 8–10 days after plating; and they metabolise the procarcinogen
N
-2-acetylaminofluorene (AAF) into a proximate form,
N
-hydroxy-AAF (ref. 5).</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/267058a0</identifier><identifier>PMID: 558527</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>2-Acetylaminofluorene - metabolism ; Albumins - physiology ; Animals ; Arginine - pharmacology ; Cell Division - drug effects ; Cells, Cultured - metabolism ; Cells, Cultured - physiology ; Fluorenes - metabolism ; Humanities and Social Sciences ; Hydrocortisone - pharmacology ; Inosine - pharmacology ; Insulin - pharmacology ; letter ; Liver - cytology ; Liver - metabolism ; Liver - physiology ; multidisciplinary ; Ornithine - metabolism ; Rats ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1977-05, Vol.267 (5606), p.58-61</ispartof><rights>Springer Nature Limited 1977</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2260-35136a5347988dfe4615d751fc7aa0848b3e213432754f474c6c5dedcf5e98d53</citedby><cites>FETCH-LOGICAL-c2260-35136a5347988dfe4615d751fc7aa0848b3e213432754f474c6c5dedcf5e98d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/558527$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LEFFERT, H. L.</creatorcontrib><creatorcontrib>MORAN, T.</creatorcontrib><creatorcontrib>BOORSTEIN, R.</creatorcontrib><creatorcontrib>KOCH, K. S.</creatorcontrib><title>Procarcinogen activation and hormonal control of cell proliferation in differentiated primary adult rat liver cell cultures</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>CONCERTED interactions among hormones and nutrients control animal cell proliferation
1
. For example, in the rat, insulin, glucagon, glucocorticoid, parathyroid hormone, iodothyronines and amino acids regulate normal hepatic proliferation—a prominent system for studying epithelial cell growth control
2
. Endocrine and nutritional factors also modify the efficiency with which chemicals produce experimental tumours in these animals notably, the induction of hepatomas by aromatic amines
8
. We report here observations with a novel primary monolayer adult rat liver culture system which may prove helpful towards studying these interactions under defined conditions
4
. These cells proliferate in response to hormones and nutrients; they maintain differentiated hepatic functions such as albumin and arginine biosynthesis for at least 8–10 days after plating; and they metabolise the procarcinogen
N
-2-acetylaminofluorene (AAF) into a proximate form,
N
-hydroxy-AAF (ref. 5).</description><subject>2-Acetylaminofluorene - metabolism</subject><subject>Albumins - physiology</subject><subject>Animals</subject><subject>Arginine - pharmacology</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured - metabolism</subject><subject>Cells, Cultured - physiology</subject><subject>Fluorenes - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Hydrocortisone - pharmacology</subject><subject>Inosine - pharmacology</subject><subject>Insulin - pharmacology</subject><subject>letter</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Liver - physiology</subject><subject>multidisciplinary</subject><subject>Ornithine - metabolism</subject><subject>Rats</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><recordid>eNplkEtLAzEUhYP4qlXwB7jIUhejeSddSvEFBV3oekjzqCnTpCQzgvjnjUx14-o-zncPlwPAOUbXGFF1Q4REXGm0ByaYSdEwoeQ-mCBEVIMUFcfgpJQ1QohjyY7AIeeKEzkBXy85GZ1NiGnlItSmDx-6D6m20cL3lDcp6g6aFPucOpg8NK7r4LYOwbs8oiFCG3wdXeyD7p2tetjo_Am1HboeVgx24cPl8djU3ZBdOQUHXnfFne3qFLzd373OH5vF88PT_HbRGEIEaijHVGhOmZwpZb1jAnMrOfZGao0UU0vqCKaMEsmZZ5IZYbh11njuZspyOgWXo6_JqZTsfLt7r8Wo_Umv_U2vohcjuh2WG2f_wDGuKl-NcqlCXLncrtOQa0Dlv9U3oX55Vw</recordid><startdate>19770505</startdate><enddate>19770505</enddate><creator>LEFFERT, H. L.</creator><creator>MORAN, T.</creator><creator>BOORSTEIN, R.</creator><creator>KOCH, K. S.</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19770505</creationdate><title>Procarcinogen activation and hormonal control of cell proliferation in differentiated primary adult rat liver cell cultures</title><author>LEFFERT, H. L. ; MORAN, T. ; BOORSTEIN, R. ; KOCH, K. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2260-35136a5347988dfe4615d751fc7aa0848b3e213432754f474c6c5dedcf5e98d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>2-Acetylaminofluorene - metabolism</topic><topic>Albumins - physiology</topic><topic>Animals</topic><topic>Arginine - pharmacology</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured - metabolism</topic><topic>Cells, Cultured - physiology</topic><topic>Fluorenes - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Hydrocortisone - pharmacology</topic><topic>Inosine - pharmacology</topic><topic>Insulin - pharmacology</topic><topic>letter</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Liver - physiology</topic><topic>multidisciplinary</topic><topic>Ornithine - metabolism</topic><topic>Rats</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LEFFERT, H. L.</creatorcontrib><creatorcontrib>MORAN, T.</creatorcontrib><creatorcontrib>BOORSTEIN, R.</creatorcontrib><creatorcontrib>KOCH, K. S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LEFFERT, H. L.</au><au>MORAN, T.</au><au>BOORSTEIN, R.</au><au>KOCH, K. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Procarcinogen activation and hormonal control of cell proliferation in differentiated primary adult rat liver cell cultures</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1977-05-05</date><risdate>1977</risdate><volume>267</volume><issue>5606</issue><spage>58</spage><epage>61</epage><pages>58-61</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>CONCERTED interactions among hormones and nutrients control animal cell proliferation
1
. For example, in the rat, insulin, glucagon, glucocorticoid, parathyroid hormone, iodothyronines and amino acids regulate normal hepatic proliferation—a prominent system for studying epithelial cell growth control
2
. Endocrine and nutritional factors also modify the efficiency with which chemicals produce experimental tumours in these animals notably, the induction of hepatomas by aromatic amines
8
. We report here observations with a novel primary monolayer adult rat liver culture system which may prove helpful towards studying these interactions under defined conditions
4
. These cells proliferate in response to hormones and nutrients; they maintain differentiated hepatic functions such as albumin and arginine biosynthesis for at least 8–10 days after plating; and they metabolise the procarcinogen
N
-2-acetylaminofluorene (AAF) into a proximate form,
N
-hydroxy-AAF (ref. 5).</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>558527</pmid><doi>10.1038/267058a0</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Nature (London), 1977-05, Vol.267 (5606), p.58-61 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_crossref_primary_10_1038_267058a0 |
| source | Nature |
| subjects | 2-Acetylaminofluorene - metabolism Albumins - physiology Animals Arginine - pharmacology Cell Division - drug effects Cells, Cultured - metabolism Cells, Cultured - physiology Fluorenes - metabolism Humanities and Social Sciences Hydrocortisone - pharmacology Inosine - pharmacology Insulin - pharmacology letter Liver - cytology Liver - metabolism Liver - physiology multidisciplinary Ornithine - metabolism Rats Science Science (multidisciplinary) |
| title | Procarcinogen activation and hormonal control of cell proliferation in differentiated primary adult rat liver cell cultures |
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