Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells

Co-cultivation of Agrobacterium tumefaciens with plant cells leads to the induction of circular copies of the T-DNA segment of the large tumour-inducing (Ti) plasmid in the bacterial cells. These circular molecules are presumably intermediates in DNA transfer from the A. tumefaciens genome to the pl...

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Bibliographic Details
Published in:Nature (London) 1985-01, Vol.313 (5999), p.191-196
Main Authors: Koukolíková-Nicola, Zdena, Shillito, Raymond D, Hohn, Barbara, Wang, Kan, Van Montagu, Marc, Zembryski, Patricia
Format: Article
Language:English
Subjects:
Bacterial plant pathogens
Bacteriology
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control
Genetics
Humanities and Social Sciences
Microbiology
Mission oriented research
multidisciplinary
Phytopathology. Animal pests. Plant and forest protection
Science
Science (multidisciplinary)
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Summary:Co-cultivation of Agrobacterium tumefaciens with plant cells leads to the induction of circular copies of the T-DNA segment of the large tumour-inducing (Ti) plasmid in the bacterial cells. These circular molecules are presumably intermediates in DNA transfer from the A. tumefaciens genome to the plant cells. In support of this suggestion, the junction of the T-DNA circles occurs precisely in the 25-base pair terminal sequence involved in T-DNA transfer.
ISSN:0028-0836
1476-4687
DOI:10.1038/313191a0