A saturable receptor for 32P-inositol-l,4,5-trisphosphate in hepatocytes and neutrophils

Several receptors for neurotransmitters, hormones and growth factors cause accelerated phosphodiesteratic breakdown of poly-phosphoinositides when activated 1,2 . One of the soluble products of this reaction, inositol-1,4,5-trisphosphate (Ins(l,4,5)P 3 ) is thought to act as a second messenger signa...

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Bibliographic Details
Published in:Nature (London) 1986-02, Vol.319 (6053), p.514-516
Main Authors: Spät, András, Bradford, Peter G., McKinney, Jerry S., Rubin, Ronald P., Putney, James W.
Format: Article
Language:English
Subjects:
Humanities and Social Sciences
letter
multidisciplinary
Science
Science (multidisciplinary)
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Summary:Several receptors for neurotransmitters, hormones and growth factors cause accelerated phosphodiesteratic breakdown of poly-phosphoinositides when activated 1,2 . One of the soluble products of this reaction, inositol-1,4,5-trisphosphate (Ins(l,4,5)P 3 ) is thought to act as a second messenger signalling the release of Ca 2+ from intracellular stores 3 . In support of this hypothesis, several studies have shown that Ins(l,4,5)P 3 releases sequestered Ca 2+ from permeable cells 4–6 and microsomes 7–9 . On the basis of certain structural requirements for Ca 2+ -releasing activity by inositol phosphates, it has been postulated that Ins(l,4,5)P 3 acts by binding to a specific intracellular receptor, probably on a component of the endoplasmic reticulum 10 . Here we report that 32 P-Ins(l,4,5)P 3 binds to a specific saturable site in permeabilized guinea pig hepatocytes and rabbit neutrophils, and that the properties of this binding site suggest that it is the physiological receptor for Ins(l,4,5)P 3 .
ISSN:0028-0836
1476-4687
DOI:10.1038/319514a0