Molecular Cloning and Expression of Two Forms of Human Protease Nexin I

Protease nexin I (PNI) is a potent anti–thrombin and anti–urokinase secreted by fibroblasts, and certain other extravascular cells. We describe the isolation, sequence, and expression of PNI cDNA cloned from a human foreskin fibroblast cDNA library. Two forms of PNI cDNA (designated α and β) have be...

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Bibliographic Details
Published in:Bio/Technology 1988-02, Vol.6 (2), p.172-177
Main Authors: McGrogan, Michael, Kennedy, Jackie, Ping Li, Mua, Hsu, Charles, Scott, Randy W, Simonsen, Christian C, Baker, Joffre B
Format: Article
Language:English
Subjects:
Agriculture
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Life Sciences
Methods. Procedures. Technologies
Molecular cloning
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Summary:Protease nexin I (PNI) is a potent anti–thrombin and anti–urokinase secreted by fibroblasts, and certain other extravascular cells. We describe the isolation, sequence, and expression of PNI cDNA cloned from a human foreskin fibroblast cDNA library. Two forms of PNI cDNA (designated α and β) have been identified. These differ only by the insertion of three nucleotides into the coding sequence, and apparently arise by utilization of an alternative splice acceptor site in the PNI gene. In the resulting proteins, αPNI contains an arg residue at position 310 where βPNI contains thr–gly residues. Both forms have been expressed in mammalian cells and inhibit thrombin and urokinase. The amino acid sequence of βPNI is identical to that of a recently described glial–derived neurite promoting factor.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0288-172