Monoclonal Antibodies Specific for Human IgE-Producing B Cells: A Potential Therapeutic for IgE-Mediated Allergic Diseases

Monoclonal antibodies (MAbs) specific for surface antigens of lymphocytes are being used to target and deplete tumorous or normal lymphocytes in vivo . Here, we report evidence for the existence of antigenic epitopes on IgE that are accessible on IgE-secreting B cells but not on other cells bearing...

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Bibliographic Details
Published in:Bio/Technology 1990-02, Vol.8 (2), p.122-126
Main Authors: Chang, Tse Wen, Davis, Frances M, Sun, Nai-Chau, Sun, Cecily R. Y, MacGlashan, Donald W, Hamilton, Robert G
Format: Article
Language:English
Subjects:
Agriculture
Animals
Antibodies, Anti-Idiotypic - immunology
Antibodies, Anti-Idiotypic - therapeutic use
Antibodies, immunoglobulins
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - therapeutic use
Antibody Specificity
Antigens, Differentiation, B-Lymphocyte - metabolism
B-Lymphocytes - immunology
B-Lymphocytes - secretion
Basophils - secretion
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Histamine Release
Humans
Hypersensitivity, Immediate - immunology
Hypersensitivity, Immediate - therapy
Immunoglobulin E - immunology
Immunoglobulin E - secretion
Immunoglobulin E - therapeutic use
Immunotherapy
Life Sciences
Mice
Molecular immunology
Monoclonal antibodies
Receptors, Fc - metabolism
Receptors, IgE
research-paper
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Summary:Monoclonal antibodies (MAbs) specific for surface antigens of lymphocytes are being used to target and deplete tumorous or normal lymphocytes in vivo . Here, we report evidence for the existence of antigenic epitopes on IgE that are accessible on IgE-secreting B cells but not on other cells bearing IgE. Among 42 murine MAbs specific for human IgE, two were shown by fluorescence flow cytometric analyses to bind to IgE-secreting cell lines but not to IgE bound to high-affinity IgE. Fc receptors (Fc∈RI) on basophils or low-affinity IgE receptors (Fc∈RII) on other cell types. Neither could they induce histamine release from basophils of various donors even under very permissive conditions. These antibodies may be useful for targeting IgE-secreting B cells in patients suffering from IgE-mediated allergies.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0290-122