Kluyveromyces as a host for heterologous gene expression: expression and secretion of prochymosin

We have developed the yeast Kluyveromyces lactis as a host organism for the production of the milk-clotting enzyme chymosin. In contrast to Saccharomyces cerevisiae , we found that this yeast is capable of the synthesis and secretion of fully active prochymosin. Various signal sequences could be use...

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Bibliographic Details
Published in:Bio/Technology 1990-02, Vol.8 (2), p.135-139
Main Authors: Berg, J.A. van den (Royal Gist-brocades, The Netherlands), Laken, K.J. van der, Van Ooyen, A.J.J, Renniers, T.C.H.M, Rietveld, K, Schaap, A, Brake, A.J, Bishop, R.J, Schultz, K, Moyer, D
Format: Article
Language:English
Subjects:
Agriculture
Amino Acid Sequence
Animals
Base Sequence
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
Biotechnology
Cattle
CHYMOSIN
Chymosin - biosynthesis
Chymosin - genetics
Chymosin - secretion
CHYMOSINE
Cloning, Molecular
Enzyme Precursors - biosynthesis
Enzyme Precursors - genetics
Enzyme Precursors - secretion
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetic engineering
Genetic technics
KLUYVEROMYCES
Kluyveromyces - genetics
Kluyveromyces - metabolism
KLUYVEROMYCES LACTIS
Life Sciences
Methods. Procedures. Technologies
Molecular Sequence Data
Plasmids
Protein Processing, Post-Translational
Protein Sorting Signals - genetics
Protein Sorting Signals - physiology
PROTEINAS
PROTEINE
PROTEINS
QUIMOSINA
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - secretion
research-paper
Saccharomyces cerevisiae - genetics
Saccharomycetales - genetics
SECRECION
SECRETION
Species Specificity
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Summary:We have developed the yeast Kluyveromyces lactis as a host organism for the production of the milk-clotting enzyme chymosin. In contrast to Saccharomyces cerevisiae , we found that this yeast is capable of the synthesis and secretion of fully active prochymosin. Various signal sequences could be used to efficiently direct the secretion of prochymosin in Kluyveromyces , but not in S. cerevisiae . We conclude that the efficient synthetic and secretory capacity of this heterologous protein is a property of the yeast Kluyveromyces . These results have led to the development of a large scale production process for chymosin.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0290-135