An Allosteric Hammerhead Ribozyme

We have constructed an RNA molecule containing a hammerhead ribozyme that is under allosteric control. In the inactive state, the RNA enzyme is unable to cleave a suitable substrate.The formation of the active state of the ribozyme is triggered by a specific interaction with a DNA oligonucleotide ef...

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Bibliographic Details
Published in:Bio/Technology 1995-02, Vol.13 (2), p.161-164
Main Authors: Porta, Helena, Lizardi, Paul M
Format: Article
Language:English
Subjects:
Agriculture
Allosteric Regulation
Base Sequence
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Drug Design
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Life Sciences
Methods. Procedures. Technologies
Molecular Sequence Data
Nucleic Acid Conformation
Oligodeoxyribonucleotides - metabolism
Polymerase Chain Reaction
Protein engineering
RNA, Catalytic - biosynthesis
RNA, Catalytic - chemistry
RNA, Catalytic - metabolism
Templates, Genetic
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Summary:We have constructed an RNA molecule containing a hammerhead ribozyme that is under allosteric control. In the inactive state, the RNA enzyme is unable to cleave a suitable substrate.The formation of the active state of the ribozyme is triggered by a specific interaction with a DNA oligonucleotide effector that is complementary to a single-stranded loop in the RNA enzyme molecule.Other DNA or RNA molecules containing unrelated nucleotide sequences do not function as allosteric effectors. This work demonstrates the feasibility of designing RNA enzymes that are specifically activated in response to an artificially designed molecular recognition event. Such enzymes may have practical applications.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0295-161