Expression in Escherichia Coli of an Epitope of the Glycoprotein of Infectious Hematopoietic Necrosis Virus Protects Against Viral Challenge

Plasmid vectors were constructed that expressed an antigenic determinant of the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV) as a fusion protein with the trp E protein of Escherichia coli . Insertion of Sau3AI fragments from the IHNV glycoprotein gene into trp E expression pla...

Full description

Saved in:
Bibliographic Details
Published in:Bio/Technology 1988-03, Vol.6 (3), p.295-300
Main Authors: Gilmore, Robert D, Engelking, H. Mark, Manning, D. Scott, Leong, JoAnn C
Format: Article
Language:English
Subjects:
Agriculture
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Life Sciences
research-paper
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plasmid vectors were constructed that expressed an antigenic determinant of the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV) as a fusion protein with the trp E protein of Escherichia coli . Insertion of Sau3AI fragments from the IHNV glycoprotein gene into trp E expression plasmids led to a fusion protein containing a hydrophilic segment of 104 amino acids from the middle portion of the viral glycoprotein. Although this region of the glycoprotein contains three N–linked glycosylation sites and is probably glycosylated in the virus, it is not glycosylated in bacteria. Nonetheless, immunization trials in fish with the crude bacterial lysate containing the fusion protein showed that the trp E–glycoprotein fusion protein produced in bacteria induced protective immunity.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0388-295