Enzyme Membrane Immunoassay (EMIA)

Homogeneous enzyme membrane immunoassays (EMIAs) have been developed for thyroxine (T 4 ) and human immuno-globulin G (IgG). Liposomes tagged with T 4 and containing alkaline phosphatase were used as a model system for optimization of (a) liposome membrane attack in the presence of T 4 antiserum and...

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Bibliographic Details
Published in:Bio/Technology 1984-04, Vol.2 (4), p.349-355
Main Authors: Braman, J. C, Broeze, R. J, Bowden, D. W, Myles, A, Fulton, T. R, Rising, M, Thurston, J, Cole, F. X, Vovis, G. F
Format: Article
Language:English
Subjects:
Agriculture
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Life Sciences
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Summary:Homogeneous enzyme membrane immunoassays (EMIAs) have been developed for thyroxine (T 4 ) and human immuno-globulin G (IgG). Liposomes tagged with T 4 and containing alkaline phosphatase were used as a model system for optimization of (a) liposome membrane attack in the presence of T 4 antiserum and guinea pig complement and (b) subsequent measurement of unmasked enzyme activity. Using a one-incubation-step format, the concentration of T 4 in human serum samples was measured. Alkaline phosphatase activity was inversely proportional to the concentration of T 4 in the sample. The T 4 values obtained by EMIA correlated well with values obtained by radioimmunoassay (r = 0.96). Likewise, there was an excellent correlation between IgG values obtained by EMIA and radialimmuno-diffusion or nephelometry (r = 0.99).
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0484-349