Crystallization of a lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium

The major lignin peroxidase from carbon limited cultures of the white–rot fungus Phanerochaete chrysosporium was purified by isoelectric focusing and crystallized by the hanging drop method. The nature of the crystalline material as lignin peroxidase was established by solid–state micro–spectrophoto...

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Bibliographic Details
Published in:Bio/Technology 1988-05, Vol.6 (5), p.571-573
Main Authors: Troller, J, Smit, J.D.G, Leisola, M.S.A, Kallen, J, Winterhalter, K.H, Fiechter, A
Format: Article
Language:English
Subjects:
Agriculture
Agronomy. Soil science and plant productions
Bioinformatics
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Chemical, physicochemical, biochemical and biological properties
ENFERMEDADES FUNGOSAS
Fundamental and applied biological sciences. Psychology
Life Sciences
LIGNINAS
LIGNINE
LIGNINS
Mission oriented research
MYCOSE
MYCOSES
PEROXIDASAS
PEROXIDASES
PEROXYDASE
Physics, chemistry, biochemistry and biology of agricultural and forest soils
Physiology and metabolism
research-paper
Soil science
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Summary:The major lignin peroxidase from carbon limited cultures of the white–rot fungus Phanerochaete chrysosporium was purified by isoelectric focusing and crystallized by the hanging drop method. The nature of the crystalline material as lignin peroxidase was established by solid–state micro–spectrophotometry and activity measurements on redissolved crystals. The ortho–rhombic, brown crystals had space group P2 1 2 1 2 1 and four lignin peroxidase molecules per unit cell.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0588-571