Using Antisense RNA to Study Gene Function: Inhibition of Carotenoid Biosynthesis in Transgenic Tomatoes

Transgenic tomato plants expressing antisense RNA to a ripening related gene (pTOM5) have yellow ripening fruit and pale colored flowers. The yellow fruit color is correlated with a severe reduction in the level of the pTOMS gene mRNA during fruit ripening. The level of carotenoids in ripening fruit...

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Bibliographic Details
Published in:Bio/Technology 1991-07, Vol.9 (7), p.635-639
Main Authors: Bird, Colin R, Ray, John A, Fletcher, Jonathon D, Boniwell, Jeremy M, Bird, Alison S, Teulieres, Chantal, Blain, Ian, Bramley, Peter M, Schuch, Wolfgang
Format: Article
Language:English
Subjects:
Agriculture
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Fundamental and applied biological sciences. Psychology
Gene expression
Life Sciences
Molecular and cellular biology
Molecular genetics
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Summary:Transgenic tomato plants expressing antisense RNA to a ripening related gene (pTOM5) have yellow ripening fruit and pale colored flowers. The yellow fruit color is correlated with a severe reduction in the level of the pTOMS gene mRNA during fruit ripening. The level of carotenoids in ripening fruit from selected transgenic plants showing yellow fruit was reduced by more than 97 percent. In addition, the carotenoid lycopene, which is primarily responsible for the red color of ripening fruit, was reduced to undetectable levels (
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0791-635