Induction of human tissue plasminogen activator in the mammary gland of transgenic goats

• Published in: Bio/Technology 1994-07, Vol.12 (7), p.699-702
• Main Authors: Ebert, K.M, DiTullio, P, Barry, C.A, Schindler, J.E, Ayres, S.L, Smith, T.E, Pellerin, L.J, Meade, H.M, Denman, J, Roberts, B
• Format: Article
• Language: English
• Subjects: ACTIVADOR PLASMINOGENO; ACTIVATEUR DE PLASMINOGENE; ADN RECOMBINADO; ADN RECOMBINE; Agriculture; ANIMAL TRANSGENIQUE; ANIMALES TRANSGENICOS; Animals; Animals, Genetically Modified; BETA-CASEIN; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; CAPRIN; CAPRINOS; CASEIN; CASEINA; CASEINE; Caseins - genetics; COMPLEMENTARY DNA; DNA, Complementary - genetics; Estradiol - pharmacology; EXPRESION GENICA; EXPRESSION DES GENES; Female; Fundamental and applied biological sciences. Psychology; GENE; GENE EXPRESSION; GENERO HUMANO; GENES; Genetic engineering; Genetic technics; GENRE HUMAIN; GLANDE MAMMAIRE; GLANDULAS MAMARIAS; GOATS; Humans; LACTACION; LACTATION; Lactation - genetics; Life Sciences; Male; MAMMARY GLANDS; Mammary Glands, Animal - metabolism; MANKIND; Methods. Procedures. Technologies; PLASMINOGEN ACTIVATOR; Progesterone - pharmacology; PROTEINAS; PROTEINE; PROTEINS; RECOMBINANT DNA; Recombinant Fusion Proteins; SECRECION; SECRETION; STRUCTURAL GENES; Tissue Plasminogen Activator - genetics; TRANSGENIC ANIMALS; Transgenic animals and transgenic plants
• ISSN: 0733-222X; 1087-0156; 2331-3684; 1546-1696
• DOI: 10.1038/nbt0794-699

Three transgenic females from a first generation transgenic male were induced to lactate between 11 and 12 months of age using a series of estrogen and progesterone injections. The milk contained human longer acting tissue plasminogen activator (LAtPA) at comparable concentrations (1–3 mg/ml) as occurred in the original founder female. In addition, the transgenic male was induced with a hormonal regime and was shown to produce 0.85 mg/ml of LAtPA. Milk protein gels indicated that the milk products (casein, IgG) were essentially normal. These experiments show that expression data for this vector can be evaluated in a shorter period of time in dairy goats than would be required through normal gestation and lactation schedules and can be used to identify the relative expression of transgenes in mammary tissue that would occur during normal lactation.