Regulation of the Chelatin Promoter During the Expression of Human Serum Albumin or Yeast Phosphoglycerate Kinase in Yeast

• Published in: Bio/Technology 1986-08, Vol.4 (8), p.726-730
• Main Authors: Etcheverry, T, Forrester, W, Hitzeman, R
• Format: Article
• Language: English
• Subjects: Agriculture; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Life Sciences; Methods. Procedures. Technologies; research-paper; Vectors (cloning, transfer, expression). Insertion sequences and transposons
• ISSN: 0733-222X; 1087-0156; 2331-3684; 1546-1696
• DOI: 10.1038/nbt0886-726

The promoter region from the yeast chelatin gene was utilized to direct the expression of a heterologous and a homologous gene in yeast. Induction of both human serum albumin and yeast phosphoglycerate kinase was regulated by the presence of copper in the growth media. A promoter fragment containing 450 bp of DNA upstream of the chelatin translational initiation codon was sufficient for inducible gene expression. Truncation of the chelatin promoter to 300 bp from the translational start site resulted in high level constitutive expression, defining a region necessary for copper induction. Deletion of the chromosomal chelatin locus did not change the regulatory properties of the plasmid promoter, suggesting that a direct autoregulatory model is not sufficient to explain chelatin gene regulation.