Gene Transfer to Tumor-Infiltrating Lymphocytes and other Mammalian Somatic Cells by Microprojectile Bombardment

We demonstrate the application of particle delivery to the transformation of mammalian somatic cells. Two mouse T–lymphocyte cell lines and Chinese hamster ovary (CHO) cells were transformed transiently with the RSV–ADH (alcohol dehydrogenase) gene. Stable gene transfer was also demonstrated in CHO...

Full description

Saved in:
Bibliographic Details
Published in:Bio/Technology 1992-09, Vol.10 (9), p.1036-1040
Main Author: Fitzpatrick-McElligott, Sandra
Format: Article
Language:English
Subjects:
Agriculture
Air Pressure
Animals
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cell Line
Cell Survival
CHO Cells
Cricetinae
Fundamental and applied biological sciences. Psychology
Gene Expression
Genetic Markers
Industrial applications and implications. Economical aspects
Life Sciences
Lymphocytes, Tumor-Infiltrating - metabolism
Mice
research-paper
Transfection - methods
Transformation, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We demonstrate the application of particle delivery to the transformation of mammalian somatic cells. Two mouse T–lymphocyte cell lines and Chinese hamster ovary (CHO) cells were transformed transiently with the RSV–ADH (alcohol dehydrogenase) gene. Stable gene transfer was also demonstrated in CHO cells at a frequency of 6 × 10 −4 and in T–cells at a frequency of 1 × 10 −4 , using β –Gal/neomycin–resistance gene constructs. The helium gas acceleration mechanism (PDS 1000/He) and particle delivery method allowed better velocity control and particle dispersion than the gunpowder driven instrument. These improvements have increased cell viability, transformation efficiency, and cellular targets.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0992-1036