"Primatization" of Recombinant Antibodies for Immunotherapy of Human Diseases: A Macaque/Human Chimeric Antibody Against Human CD4

Immunoglobulin variable region genes from non-human primates, cynomolgus macaques, were shown to have 85%–98% homology with human immunoglobulin sequences and yet macaques are phylogenetically distant enough to respond against conserved human antigens. Immunoglobulin genes were isolated from monkeys...

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Bibliographic Details
Published in:Bio/Technology 1992-11, Vol.10 (11), p.1455-1460
Main Authors: Newman, Roland, Alberts, James, Anderson, Darrell, Carner, Kristin, Heard, Cheryl, Norton, Frank, Raab, Ron, Reff, Mitchell, Shuey, Steve, Hanna, Nabil
Format: Article
Language:English
Subjects:
Agriculture
Animals
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal - therapeutic use
Base Sequence
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
CD4 Antigens - immunology
Cloning, Molecular
Humans
Immunoglobulins - genetics
Immunotherapy - methods
Life Sciences
Macaca fascicularis
Molecular Sequence Data
Recombinant Proteins - analysis
Recombinant Proteins - therapeutic use
research-paper
Sequence Alignment
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
T-Lymphocytes - immunology
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Summary:Immunoglobulin variable region genes from non-human primates, cynomolgus macaques, were shown to have 85%–98% homology with human immunoglobulin sequences and yet macaques are phylogenetically distant enough to respond against conserved human antigens. Immunoglobulin genes were isolated from monkeys immunized with human CD4 antigen and a human/monkey chimeric anti-CD4 antibody with 91–92% homology to human immunoglobulin framework regions was cloned and expressed. The antibody has an apparent affinity of 3.2 × 10 −11 M and exhibits potent immunosuppressive properties in vitro.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt1192-1455