High level expression of recombinant genes in Aspergillus oryzae

We have modified a method for transformation of Aspergillus nidulans 1 to work for Aspergillus oryzae using selection for the amdS and argB genes from Aspergillus nidulans . To direct the expression of recombinant genes in A. oryzae we have used an alpha-amylase promoter cloned from a high yielding...

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Bibliographic Details
Published in:Bio/Technology 1988-12, Vol.6 (12), p.1419-1422
Main Authors: Christensen, T, Woeldike, H, Boel, E, Mortensen, S.B, Hjortshoej, K, Thim, L, Hansen, M.T
Format: Article
Language:English
Subjects:
Agriculture
ASPERGILLUS
ASPERGILLUS NIDULANS
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
FOOD TECHNOLOGY
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetic engineering
Genetic technics
GENETIC TRANSFORMATION
Life Sciences
Methods. Procedures. Technologies
PROTEINAS
PROTEINE
PROTEINS
RECOMBINACION
RECOMBINAISON
RECOMBINATION
research-paper
TECHNOLOGIE ALIMENTAIRE
TECNOLOGIA DE LOS ALIMENTOS
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:We have modified a method for transformation of Aspergillus nidulans 1 to work for Aspergillus oryzae using selection for the amdS and argB genes from Aspergillus nidulans . To direct the expression of recombinant genes in A. oryzae we have used an alpha-amylase promoter cloned from a high yielding strain of A. oryzae . The amounts of heterologous protein obtained make this system attractive for even moderately priced industrial enzymes. As an example, the Rhizomucor miehei derived aspartic proteinase was secreted with yields in excess of three grams per liter. The proteinase was slightly overglycosylated, which did not, however, alter the specific activity of the enzyme.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt1288-1419