Annexin‐1 peptide Anx‐1 2–26 protects adult rat cardiac myocytes from cellular injury induced by simulated ischaemia

The anti‐inflammatory properties of annexin‐1 peptides have been largely ascribed to their powerful antineutrophil actions in vivo . We have recently reported that the N‐terminal fragment of annexin‐1, Anx‐1 2–26 , preserves contractile function of cardiac muscle in vitro . The aim of the present st...

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Bibliographic Details
Published in:British journal of pharmacology 2009-01, Vol.145 (4), p.495-502
Main Authors: Ritchie, Rebecca H, Gordon, Jennifer M, Woodman, Owen L, Cao, Anh H, Dusting, Gregory J
Format: Article
Language:English
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Summary:The anti‐inflammatory properties of annexin‐1 peptides have been largely ascribed to their powerful antineutrophil actions in vivo . We have recently reported that the N‐terminal fragment of annexin‐1, Anx‐1 2–26 , preserves contractile function of cardiac muscle in vitro . The aim of the present study was to determine if Anx‐1 2–26 elicits protective actions specifically on the cardiac myocyte (in the absence of neutrophils), using a model of metabolic inhibition to simulate ischaemia. Metabolic inhibition of cardiac myocytes (4 h incubation at 37°C in HEPES‐containing buffer supplemented with 2‐deoxy‐ D ‐glucose, D , L ‐lactic acid and pH adjusted to 6.5) followed by 2.5 h recovery in normal medium markedly increased creatine kinase (CK) and lactate dehydrogenase (LDH) levels by 179±39 and 26±7 IU L −1 (both n =40, P
ISSN:0007-1188
1476-5381
DOI:10.1038/sj.bjp.0706211