Preconcentration and determination of manganese in biological samples by dual-cloud point extraction coupled with flame atomic absorption spectrometry

• Published in: Journal of analytical atomic spectrometry 2014-01, Vol.29 (12), p.2349-2355
• Main Authors: Arain, Mariam S, Kazi, Tasneem G, Afridi, Hassan I, Arain, Salma A, Ali, Jamshed, Naeemullah, Arain, Sadaf S, Panhwar, Abdul Haleem, Shanker, Bhawani
• Format: Article
• Language: English
• Subjects: Atomic absorption analysis; Biological; Extraction; Hair; Joining; Manganese; Mathematical analysis; Spectrometry
• ISSN: 0267-9477; 1364-5544
• DOI: 10.1039/c4ja00266k

An efficient, innovative preconcentration method using dual-cloud point extraction (d-CPE) was developed for the analysis of trace levels of manganese (Mn 2+ ) in biological samples (scalp hair) prior to coupling with flame atomic absorption spectrometry (FAAS). In the first step of d-CPE, Mn 2+ was complexed with 1-(2-pyridylazo)-2-naphthol (PAN), which was subsequently entrapped in a nonionic surfactant (Triton X-114). In the second step, the entrapped metal complex in the surfactant-rich phase was treated with aqueous nitric acid solution and heated; the studied metal ions were back-extracted again into aqueous phase. The aqueous phase was subjected to FAAS using conventional nebulization. The validity of the proposed method was verified by the analysis of Mn 2+ in a certified sample of scalp hair (CRM) by both d-CPE and conventional CPE procedures. The enhancement factor of Mn 2+ was found to be 46. The proposed method was successfully applied to the determination of Mn 2+ in acid-digested scalp hair samples of Parkinson's patients and healthy referents. An efficient, innovative preconcentration method using dual-cloud point extraction (d-CPE) was developed for the analysis of trace levels of manganese (Mn 2+ ) in biological samples (scalp hair) prior to coupling with flame atomic absorption spectrometry (FAAS).