A bacterial DNA quadruplex with exceptional K + selectivity and unique structural polymorphism

The G-rich sequence d[(G 4 CT) 3 G 4 ] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum . We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectrosc...

Full description

Saved in:
Bibliographic Details
Published in:Chemical science (Cambridge) 2014, Vol.5 (7), p.2809-2818
Main Authors: Rehm, Charlotte, Holder, Isabelle T., Groß, Andreas, Wojciechowski, Filip, Urban, Maximilian, Sinn, Malte, Drescher, Malte, Hartig, Jörg S.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c308t-5c44a267444b1c59dd5f2af62d9abbbc36aa26922802e3f0f1b36844b6bab4523
cites cdi_FETCH-LOGICAL-c308t-5c44a267444b1c59dd5f2af62d9abbbc36aa26922802e3f0f1b36844b6bab4523
container_end_page 2818
container_issue 7
container_start_page 2809
container_title Chemical science (Cambridge)
container_volume 5
creator Rehm, Charlotte
Holder, Isabelle T.
Groß, Andreas
Wojciechowski, Filip
Urban, Maximilian
Sinn, Malte
Drescher, Malte
Hartig, Jörg S.
description The G-rich sequence d[(G 4 CT) 3 G 4 ] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum . We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectroscopy, EPR spectroscopy, analytical ultracentrifugation, and EMSA, we demonstrate that d[(G 4 CT) 3 G 4 ] displays unique features among the many G-quadruplex-forming sequences studied so far. To our knowledge d[(G 4 CT) 3 G 4 ] shows a so far unprecedented selectivity for K + with even high concentrations of Na + unable to induce pronounced G-quadruplex formation. A remarkable continuous and complete transition from an anti-parallel, monomolecular structure into a tetrameric, parallel conformation is observed upon increasing K + -concentrations. Furthermore we investigate the effects of cation selectivity, quadruplex loop composition and length as well as G-tract length on quadruplex conversion.
doi_str_mv 10.1039/C4SC00440J
format article
fullrecord <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_1039_C4SC00440J</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1039_C4SC00440J</sourcerecordid><originalsourceid>FETCH-LOGICAL-c308t-5c44a267444b1c59dd5f2af62d9abbbc36aa26922802e3f0f1b36844b6bab4523</originalsourceid><addsrcrecordid>eNpFkDtPwzAAhC0EElXpwi_wDAr43WSMApRHBQOwEtmOrRolTeoHNP-eIBDccid9pxsOgFOMLjCixWXFniuEGEP3B2BGEMOZ4LQ4_MsEHYNFCO9oEqWYk-UMvJVQSR2Nd7KFV48l3CXZ-DS0Zg8_XdxAs9dmiK7fTvwBnsNgWqOj-3BxhHLbwLR1u2RgiD7pmPzUGvp27Ho_bFzoTsCRlW0wi1-fg9eb65fqNls_re6qcp1pivKYcc2YJGLJGFNY86JpuCXSCtIUUimlqZATLgjJETHUIosVFflUFkoqxgmdg7OfXe37ELyx9eBdJ_1YY1R_n1P_n0O_AMjEV9s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>A bacterial DNA quadruplex with exceptional K + selectivity and unique structural polymorphism</title><source>PubMed Central</source><source>Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list)</source><creator>Rehm, Charlotte ; Holder, Isabelle T. ; Groß, Andreas ; Wojciechowski, Filip ; Urban, Maximilian ; Sinn, Malte ; Drescher, Malte ; Hartig, Jörg S.</creator><creatorcontrib>Rehm, Charlotte ; Holder, Isabelle T. ; Groß, Andreas ; Wojciechowski, Filip ; Urban, Maximilian ; Sinn, Malte ; Drescher, Malte ; Hartig, Jörg S.</creatorcontrib><description>The G-rich sequence d[(G 4 CT) 3 G 4 ] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum . We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectroscopy, EPR spectroscopy, analytical ultracentrifugation, and EMSA, we demonstrate that d[(G 4 CT) 3 G 4 ] displays unique features among the many G-quadruplex-forming sequences studied so far. To our knowledge d[(G 4 CT) 3 G 4 ] shows a so far unprecedented selectivity for K + with even high concentrations of Na + unable to induce pronounced G-quadruplex formation. A remarkable continuous and complete transition from an anti-parallel, monomolecular structure into a tetrameric, parallel conformation is observed upon increasing K + -concentrations. Furthermore we investigate the effects of cation selectivity, quadruplex loop composition and length as well as G-tract length on quadruplex conversion.</description><identifier>ISSN: 2041-6520</identifier><identifier>EISSN: 2041-6539</identifier><identifier>DOI: 10.1039/C4SC00440J</identifier><language>eng</language><ispartof>Chemical science (Cambridge), 2014, Vol.5 (7), p.2809-2818</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c308t-5c44a267444b1c59dd5f2af62d9abbbc36aa26922802e3f0f1b36844b6bab4523</citedby><cites>FETCH-LOGICAL-c308t-5c44a267444b1c59dd5f2af62d9abbbc36aa26922802e3f0f1b36844b6bab4523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Rehm, Charlotte</creatorcontrib><creatorcontrib>Holder, Isabelle T.</creatorcontrib><creatorcontrib>Groß, Andreas</creatorcontrib><creatorcontrib>Wojciechowski, Filip</creatorcontrib><creatorcontrib>Urban, Maximilian</creatorcontrib><creatorcontrib>Sinn, Malte</creatorcontrib><creatorcontrib>Drescher, Malte</creatorcontrib><creatorcontrib>Hartig, Jörg S.</creatorcontrib><title>A bacterial DNA quadruplex with exceptional K + selectivity and unique structural polymorphism</title><title>Chemical science (Cambridge)</title><description>The G-rich sequence d[(G 4 CT) 3 G 4 ] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum . We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectroscopy, EPR spectroscopy, analytical ultracentrifugation, and EMSA, we demonstrate that d[(G 4 CT) 3 G 4 ] displays unique features among the many G-quadruplex-forming sequences studied so far. To our knowledge d[(G 4 CT) 3 G 4 ] shows a so far unprecedented selectivity for K + with even high concentrations of Na + unable to induce pronounced G-quadruplex formation. A remarkable continuous and complete transition from an anti-parallel, monomolecular structure into a tetrameric, parallel conformation is observed upon increasing K + -concentrations. Furthermore we investigate the effects of cation selectivity, quadruplex loop composition and length as well as G-tract length on quadruplex conversion.</description><issn>2041-6520</issn><issn>2041-6539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNpFkDtPwzAAhC0EElXpwi_wDAr43WSMApRHBQOwEtmOrRolTeoHNP-eIBDccid9pxsOgFOMLjCixWXFniuEGEP3B2BGEMOZ4LQ4_MsEHYNFCO9oEqWYk-UMvJVQSR2Nd7KFV48l3CXZ-DS0Zg8_XdxAs9dmiK7fTvwBnsNgWqOj-3BxhHLbwLR1u2RgiD7pmPzUGvp27Ho_bFzoTsCRlW0wi1-fg9eb65fqNls_re6qcp1pivKYcc2YJGLJGFNY86JpuCXSCtIUUimlqZATLgjJETHUIosVFflUFkoqxgmdg7OfXe37ELyx9eBdJ_1YY1R_n1P_n0O_AMjEV9s</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Rehm, Charlotte</creator><creator>Holder, Isabelle T.</creator><creator>Groß, Andreas</creator><creator>Wojciechowski, Filip</creator><creator>Urban, Maximilian</creator><creator>Sinn, Malte</creator><creator>Drescher, Malte</creator><creator>Hartig, Jörg S.</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2014</creationdate><title>A bacterial DNA quadruplex with exceptional K + selectivity and unique structural polymorphism</title><author>Rehm, Charlotte ; Holder, Isabelle T. ; Groß, Andreas ; Wojciechowski, Filip ; Urban, Maximilian ; Sinn, Malte ; Drescher, Malte ; Hartig, Jörg S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c308t-5c44a267444b1c59dd5f2af62d9abbbc36aa26922802e3f0f1b36844b6bab4523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rehm, Charlotte</creatorcontrib><creatorcontrib>Holder, Isabelle T.</creatorcontrib><creatorcontrib>Groß, Andreas</creatorcontrib><creatorcontrib>Wojciechowski, Filip</creatorcontrib><creatorcontrib>Urban, Maximilian</creatorcontrib><creatorcontrib>Sinn, Malte</creatorcontrib><creatorcontrib>Drescher, Malte</creatorcontrib><creatorcontrib>Hartig, Jörg S.</creatorcontrib><collection>CrossRef</collection><jtitle>Chemical science (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rehm, Charlotte</au><au>Holder, Isabelle T.</au><au>Groß, Andreas</au><au>Wojciechowski, Filip</au><au>Urban, Maximilian</au><au>Sinn, Malte</au><au>Drescher, Malte</au><au>Hartig, Jörg S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A bacterial DNA quadruplex with exceptional K + selectivity and unique structural polymorphism</atitle><jtitle>Chemical science (Cambridge)</jtitle><date>2014</date><risdate>2014</risdate><volume>5</volume><issue>7</issue><spage>2809</spage><epage>2818</epage><pages>2809-2818</pages><issn>2041-6520</issn><eissn>2041-6539</eissn><abstract>The G-rich sequence d[(G 4 CT) 3 G 4 ] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum . We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectroscopy, EPR spectroscopy, analytical ultracentrifugation, and EMSA, we demonstrate that d[(G 4 CT) 3 G 4 ] displays unique features among the many G-quadruplex-forming sequences studied so far. To our knowledge d[(G 4 CT) 3 G 4 ] shows a so far unprecedented selectivity for K + with even high concentrations of Na + unable to induce pronounced G-quadruplex formation. A remarkable continuous and complete transition from an anti-parallel, monomolecular structure into a tetrameric, parallel conformation is observed upon increasing K + -concentrations. Furthermore we investigate the effects of cation selectivity, quadruplex loop composition and length as well as G-tract length on quadruplex conversion.</abstract><doi>10.1039/C4SC00440J</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2041-6520
ispartof Chemical science (Cambridge), 2014, Vol.5 (7), p.2809-2818
issn 2041-6520
2041-6539
language eng
recordid cdi_crossref_primary_10_1039_C4SC00440J
source PubMed Central; Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list)
title A bacterial DNA quadruplex with exceptional K + selectivity and unique structural polymorphism
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T07%3A35%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20bacterial%20DNA%20quadruplex%20with%20exceptional%20K%20+%20selectivity%20and%20unique%20structural%20polymorphism&rft.jtitle=Chemical%20science%20(Cambridge)&rft.au=Rehm,%20Charlotte&rft.date=2014&rft.volume=5&rft.issue=7&rft.spage=2809&rft.epage=2818&rft.pages=2809-2818&rft.issn=2041-6520&rft.eissn=2041-6539&rft_id=info:doi/10.1039/C4SC00440J&rft_dat=%3Ccrossref%3E10_1039_C4SC00440J%3C/crossref%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c308t-5c44a267444b1c59dd5f2af62d9abbbc36aa26922802e3f0f1b36844b6bab4523%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true