A fast click-slow release strategy towards the HPLC-free synthesis of RNA

A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels-Alder reaction between trans -cyclooctene and tetrazine, termed 'click-to-release'...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (7), p.145-148
Main Authors: Agustin, E, Asare Okai, P. N, Khan, I, Miller, M. R, Wang, R, Sheng, J, Royzen, M
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid - methods
Click Chemistry
RNA - chemical synthesis
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Summary:A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels-Alder reaction between trans -cyclooctene and tetrazine, termed 'click-to-release'. The strategy has been applied towards the synthesis and purification of a model hairpin RNA strand, as well as a 34 nt long aptamer. A general strategy for the purification of oligonucleotides synthesized by solid phase synthesis is described.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc05392g