Loading…
Delivery of AIB1 siRNA by Ca 2+ /PEI/heparin composite nanoparticles effectively inhibits the growth of human breast cancer
Here, a novel carrier fabricated by the interaction of negatively charged heparin and positively charged PEI and Ca was investigated to deliver AIB1 siRNA into breast cancer cells both in vitro and in vivo. Ca /PEI/heparin nanoparticles were prepared by simply mixing heparin, PEI and CaCl aqueous so...
Saved in:
| Published in: | Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2015-10, Vol.3 (38), p.7623-7630 |
|---|---|
| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c996-2a0aa3b2032e1437eb1ff4f897159ddb0e0b72df2f641868a88ea426cdee09593 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c996-2a0aa3b2032e1437eb1ff4f897159ddb0e0b72df2f641868a88ea426cdee09593 |
| container_end_page | 7630 |
| container_issue | 38 |
| container_start_page | 7623 |
| container_title | Journal of materials chemistry. B, Materials for biology and medicine |
| container_volume | 3 |
| creator | Cheang, T Y Xing, Z H Li, Z L Zhou, H Y Wei, J H Zhou, X Xu, A W Lin, Y Wang, S M |
| description | Here, a novel carrier fabricated by the interaction of negatively charged heparin and positively charged PEI and Ca
was investigated to deliver AIB1 siRNA into breast cancer cells both in vitro and in vivo. Ca
/PEI/heparin nanoparticles were prepared by simply mixing heparin, PEI and CaCl
aqueous solution. Heparin in the Ca
/PEI/heparin nanoparticles (40.9% heparin, w/w) decreased the cytotoxicity of PEI. According to the MTT assay, Ca
/PEI/heparin NPs are superior to commercial Lipofectamine 2000 considering the safety. The Ca
/PEI/heparin NPs are able to deliver siAIB1 into breast cancer cells as effectively as Lipofectamine 2000 both in vitro and in vivo. The in vivo experiment also indicated that the NF-κB/BCL-2 signal pathway might be the downstream signal pathway of AIB1 in regulating breast cancer proliferation and progression. |
| doi_str_mv | 10.1039/c5tb01490e |
| format | article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1039_C5TB01490E</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>32262646</sourcerecordid><originalsourceid>FETCH-LOGICAL-c996-2a0aa3b2032e1437eb1ff4f897159ddb0e0b72df2f641868a88ea426cdee09593</originalsourceid><addsrcrecordid>eNo9kE1Lw0AQQBdRbKm9-ANkz0rsfiSb5NjWqoWiIj14C7ubWbOSL3ZTJfjnTa12LjMMj3d4CF1ScksJT2c66hShYUrgBI0ZiUgQRzQ5Pd7kbYSm3n-QYRIqEh6eoxFnTDARijH6voPSfoLrcWPwfL2g2NvXpzlWPV5KzG7w7GW1nhXQSmdrrJuqbbztANeyboZfZ3UJHoMxoLvBU_bY1oVVtvO4KwC_u-arK_buYlfJGisH0ndYy1qDu0BnRpYepn97grb3q-3yMdg8P6yX802g01QETBIpuWKEM6Ahj0FRY0KTpDGN0jxXBIiKWW6YESFNRCKTBGTIhM4BSBqlfIKuD1rtGu8dmKx1tpKuzyjJ9g2zZbRd_DZcDfDVAW53qoL8iP4X4z8XMmxW</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Delivery of AIB1 siRNA by Ca 2+ /PEI/heparin composite nanoparticles effectively inhibits the growth of human breast cancer</title><source>Royal Society of Chemistry</source><creator>Cheang, T Y ; Xing, Z H ; Li, Z L ; Zhou, H Y ; Wei, J H ; Zhou, X ; Xu, A W ; Lin, Y ; Wang, S M</creator><creatorcontrib>Cheang, T Y ; Xing, Z H ; Li, Z L ; Zhou, H Y ; Wei, J H ; Zhou, X ; Xu, A W ; Lin, Y ; Wang, S M</creatorcontrib><description>Here, a novel carrier fabricated by the interaction of negatively charged heparin and positively charged PEI and Ca
was investigated to deliver AIB1 siRNA into breast cancer cells both in vitro and in vivo. Ca
/PEI/heparin nanoparticles were prepared by simply mixing heparin, PEI and CaCl
aqueous solution. Heparin in the Ca
/PEI/heparin nanoparticles (40.9% heparin, w/w) decreased the cytotoxicity of PEI. According to the MTT assay, Ca
/PEI/heparin NPs are superior to commercial Lipofectamine 2000 considering the safety. The Ca
/PEI/heparin NPs are able to deliver siAIB1 into breast cancer cells as effectively as Lipofectamine 2000 both in vitro and in vivo. The in vivo experiment also indicated that the NF-κB/BCL-2 signal pathway might be the downstream signal pathway of AIB1 in regulating breast cancer proliferation and progression.</description><identifier>ISSN: 2050-750X</identifier><identifier>EISSN: 2050-7518</identifier><identifier>DOI: 10.1039/c5tb01490e</identifier><identifier>PMID: 32262646</identifier><language>eng</language><publisher>England</publisher><ispartof>Journal of materials chemistry. B, Materials for biology and medicine, 2015-10, Vol.3 (38), p.7623-7630</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c996-2a0aa3b2032e1437eb1ff4f897159ddb0e0b72df2f641868a88ea426cdee09593</citedby><cites>FETCH-LOGICAL-c996-2a0aa3b2032e1437eb1ff4f897159ddb0e0b72df2f641868a88ea426cdee09593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32262646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheang, T Y</creatorcontrib><creatorcontrib>Xing, Z H</creatorcontrib><creatorcontrib>Li, Z L</creatorcontrib><creatorcontrib>Zhou, H Y</creatorcontrib><creatorcontrib>Wei, J H</creatorcontrib><creatorcontrib>Zhou, X</creatorcontrib><creatorcontrib>Xu, A W</creatorcontrib><creatorcontrib>Lin, Y</creatorcontrib><creatorcontrib>Wang, S M</creatorcontrib><title>Delivery of AIB1 siRNA by Ca 2+ /PEI/heparin composite nanoparticles effectively inhibits the growth of human breast cancer</title><title>Journal of materials chemistry. B, Materials for biology and medicine</title><addtitle>J Mater Chem B</addtitle><description>Here, a novel carrier fabricated by the interaction of negatively charged heparin and positively charged PEI and Ca
was investigated to deliver AIB1 siRNA into breast cancer cells both in vitro and in vivo. Ca
/PEI/heparin nanoparticles were prepared by simply mixing heparin, PEI and CaCl
aqueous solution. Heparin in the Ca
/PEI/heparin nanoparticles (40.9% heparin, w/w) decreased the cytotoxicity of PEI. According to the MTT assay, Ca
/PEI/heparin NPs are superior to commercial Lipofectamine 2000 considering the safety. The Ca
/PEI/heparin NPs are able to deliver siAIB1 into breast cancer cells as effectively as Lipofectamine 2000 both in vitro and in vivo. The in vivo experiment also indicated that the NF-κB/BCL-2 signal pathway might be the downstream signal pathway of AIB1 in regulating breast cancer proliferation and progression.</description><issn>2050-750X</issn><issn>2050-7518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNo9kE1Lw0AQQBdRbKm9-ANkz0rsfiSb5NjWqoWiIj14C7ubWbOSL3ZTJfjnTa12LjMMj3d4CF1ScksJT2c66hShYUrgBI0ZiUgQRzQ5Pd7kbYSm3n-QYRIqEh6eoxFnTDARijH6voPSfoLrcWPwfL2g2NvXpzlWPV5KzG7w7GW1nhXQSmdrrJuqbbztANeyboZfZ3UJHoMxoLvBU_bY1oVVtvO4KwC_u-arK_buYlfJGisH0ndYy1qDu0BnRpYepn97grb3q-3yMdg8P6yX802g01QETBIpuWKEM6Ahj0FRY0KTpDGN0jxXBIiKWW6YESFNRCKTBGTIhM4BSBqlfIKuD1rtGu8dmKx1tpKuzyjJ9g2zZbRd_DZcDfDVAW53qoL8iP4X4z8XMmxW</recordid><startdate>20151014</startdate><enddate>20151014</enddate><creator>Cheang, T Y</creator><creator>Xing, Z H</creator><creator>Li, Z L</creator><creator>Zhou, H Y</creator><creator>Wei, J H</creator><creator>Zhou, X</creator><creator>Xu, A W</creator><creator>Lin, Y</creator><creator>Wang, S M</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20151014</creationdate><title>Delivery of AIB1 siRNA by Ca 2+ /PEI/heparin composite nanoparticles effectively inhibits the growth of human breast cancer</title><author>Cheang, T Y ; Xing, Z H ; Li, Z L ; Zhou, H Y ; Wei, J H ; Zhou, X ; Xu, A W ; Lin, Y ; Wang, S M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c996-2a0aa3b2032e1437eb1ff4f897159ddb0e0b72df2f641868a88ea426cdee09593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheang, T Y</creatorcontrib><creatorcontrib>Xing, Z H</creatorcontrib><creatorcontrib>Li, Z L</creatorcontrib><creatorcontrib>Zhou, H Y</creatorcontrib><creatorcontrib>Wei, J H</creatorcontrib><creatorcontrib>Zhou, X</creatorcontrib><creatorcontrib>Xu, A W</creatorcontrib><creatorcontrib>Lin, Y</creatorcontrib><creatorcontrib>Wang, S M</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheang, T Y</au><au>Xing, Z H</au><au>Li, Z L</au><au>Zhou, H Y</au><au>Wei, J H</au><au>Zhou, X</au><au>Xu, A W</au><au>Lin, Y</au><au>Wang, S M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Delivery of AIB1 siRNA by Ca 2+ /PEI/heparin composite nanoparticles effectively inhibits the growth of human breast cancer</atitle><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle><addtitle>J Mater Chem B</addtitle><date>2015-10-14</date><risdate>2015</risdate><volume>3</volume><issue>38</issue><spage>7623</spage><epage>7630</epage><pages>7623-7630</pages><issn>2050-750X</issn><eissn>2050-7518</eissn><abstract>Here, a novel carrier fabricated by the interaction of negatively charged heparin and positively charged PEI and Ca
was investigated to deliver AIB1 siRNA into breast cancer cells both in vitro and in vivo. Ca
/PEI/heparin nanoparticles were prepared by simply mixing heparin, PEI and CaCl
aqueous solution. Heparin in the Ca
/PEI/heparin nanoparticles (40.9% heparin, w/w) decreased the cytotoxicity of PEI. According to the MTT assay, Ca
/PEI/heparin NPs are superior to commercial Lipofectamine 2000 considering the safety. The Ca
/PEI/heparin NPs are able to deliver siAIB1 into breast cancer cells as effectively as Lipofectamine 2000 both in vitro and in vivo. The in vivo experiment also indicated that the NF-κB/BCL-2 signal pathway might be the downstream signal pathway of AIB1 in regulating breast cancer proliferation and progression.</abstract><cop>England</cop><pmid>32262646</pmid><doi>10.1039/c5tb01490e</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2050-750X |
| ispartof | Journal of materials chemistry. B, Materials for biology and medicine, 2015-10, Vol.3 (38), p.7623-7630 |
| issn | 2050-750X 2050-7518 |
| language | eng |
| recordid | cdi_crossref_primary_10_1039_C5TB01490E |
| source | Royal Society of Chemistry |
| title | Delivery of AIB1 siRNA by Ca 2+ /PEI/heparin composite nanoparticles effectively inhibits the growth of human breast cancer |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T11%3A52%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Delivery%20of%20AIB1%20siRNA%20by%20Ca%202+%20/PEI/heparin%20composite%20nanoparticles%20effectively%20inhibits%20the%20growth%20of%20human%20breast%20cancer&rft.jtitle=Journal%20of%20materials%20chemistry.%20B,%20Materials%20for%20biology%20and%20medicine&rft.au=Cheang,%20T%20Y&rft.date=2015-10-14&rft.volume=3&rft.issue=38&rft.spage=7623&rft.epage=7630&rft.pages=7623-7630&rft.issn=2050-750X&rft.eissn=2050-7518&rft_id=info:doi/10.1039/c5tb01490e&rft_dat=%3Cpubmed_cross%3E32262646%3C/pubmed_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c996-2a0aa3b2032e1437eb1ff4f897159ddb0e0b72df2f641868a88ea426cdee09593%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/32262646&rfr_iscdi=true |