Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

We use single molecule spectroscopy to study a multicomponent, competitive protein adsorption system. Fluorescently-labeled α-lactalbumin proteins are super-resolved adsorbing to cationic anion-exchange ligands in the presence of a competitor, insulin. We find that the competitor reduces the number...

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Bibliographic Details
Published in:Analyst (London) 2017-09, Vol.142 (17), p.3127-3131
Main Authors: Kisley, Lydia, Patil, Ujwal, Dhamane, Sagar, Kourentzi, Katerina, Tauzin, Lawrence J, Willson, Richard C, Landes, Christy F
Format: Article
Language:English
Subjects:
Adsorption
Anions
Fluorescence
Kinetics
Lactalbumin - analysis
Ligands
Spectrum Analysis
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Summary:We use single molecule spectroscopy to study a multicomponent, competitive protein adsorption system. Fluorescently-labeled α-lactalbumin proteins are super-resolved adsorbing to cationic anion-exchange ligands in the presence of a competitor, insulin. We find that the competitor reduces the number of binding events by blocking ligands throughout the observed measurement time while the single-site adsorption kinetics are unchanged.
ISSN:0003-2654
1364-5528
DOI:10.1039/c7an00701a