High temporal resolution delayed analysis of clinical microdialysate streams

This paper presents the use of tubing to store clinical microdialysis samples for delayed analysis with high temporal resolution, offering an alternative to traditional discrete offline microdialysis sampling. Samples stored in this way were found to be stable for up to 72 days at −80 °C. Examples o...

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Bibliographic Details
Published in:Analyst (London) 2018-01, Vol.143 (3), p.715-724
Main Authors: Gowers, S. A. N, Hamaoui, K, Cunnea, P, Anastasova, S, Curto, V. F, Vadgama, P, Yang, G.-Z, Papalois, V, Drakakis, E. M, Fotopoulou, C, Weber, S. G, Boutelle, M. G
Format: Article
Language:English
Subjects:
Chemistry
Flow velocity
Temporal resolution
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Summary:This paper presents the use of tubing to store clinical microdialysis samples for delayed analysis with high temporal resolution, offering an alternative to traditional discrete offline microdialysis sampling. Samples stored in this way were found to be stable for up to 72 days at −80 °C. Examples of how this methodology can be applied to glucose and lactate measurement in a wide range of in vivo monitoring experiments are presented. This paper presents a general model, which allows for an informed choice of tubing parameters for a given storage time and flow rate avoiding high back pressure, which would otherwise cause the microdialysis probe to leak, while maximising temporal resolution. This paper presents the use of tubing to store clinical microdialysis samples for delayed analysis with high temporal resolution, offering an alternative to traditional discrete offline microdialysis sampling. A model allowing optimal results is described.
ISSN:0003-2654
1364-5528
DOI:10.1039/c7an01209h