A simple pre-column derivatization method for the determination of mancozeb technical (fungicide) by reverse phase HPLC-UV

• Published in: Analytical methods 2017-08, Vol.9 (32), p.472-478
• Main Authors: Petha, Narendra H, Lokhande, Rama S, Seshadri, Devender T, Patil, Raju M, Bhagat, Tanaji S, Patil, Jitendra G
• Format: Article
• Language: English
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/c7ay00830a

A simple and fast pre-column derivatization high performance liquid chromatographic method for the technical analysis of mancozeb was developed and validated in the present study. The mobile phase consists of a mixture of acetonitrile and 0.1% (v/v) formic acid in the proportion 60 : 40. This was found to give a sharp peak of the S -ethyl derivative of mancozeb ( S -ethyl ethylene bis-dithiocarbamate) at a retention time of 9.12 minutes. HPLC analysis of mancozeb was carried out at a wavelength of 272 nm with a flow rate of 1.0 mL per minute. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient of 0.999 in the concentration range of 8.2 mg L −1 to 32.3 mg L −1 for the derivative of mancozeb technical. The method was validated for specificity, linearity, precision and accuracy. A simple and fast pre-column derivatization high performance liquid chromatographic method for the technical analysis of mancozeb was developed and validated in the present study.