A gold nanoparticle-modified indium tin oxide microelectrode for in-channel amperometric detection in dual-channel microchip electrophoresis

A modified indium tin oxide (ITO) microelectrode detector integrated with a dual-channel polydimethylsiloxane (PDMS) microchip is proposed for in-channel amperometric detection in microchip electrophoresis (ME). The gold nanoparticle (GNP)-modified ITO-coated poly-ethylene terephthalate film electro...

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Bibliographic Details
Published in:Analytical methods 2017, Vol.9 (29), p.4319-4326
Main Authors: Zhu, Gangzhi, Song, Qianhui, Liu, Wenfang, Yan, Xingxing, Xiao, Jian, Chen, Chuanpin
Format: Article
Language:English
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Summary:A modified indium tin oxide (ITO) microelectrode detector integrated with a dual-channel polydimethylsiloxane (PDMS) microchip is proposed for in-channel amperometric detection in microchip electrophoresis (ME). The gold nanoparticle (GNP)-modified ITO-coated poly-ethylene terephthalate film electrode was fabricated via a photolithography process and direct electrochemical deposition. This electrode fabrication method reduced the cost of this microchip a lot. The electroplating conditions for the GNP-modified ITO electrode were optimized through evaluating the effect on the detection sensitivity and separation efficiency of a dual-channel ME system. Under the optimal conditions, the performance of the new ME amperometric detection system has been demonstrated by analyzing a mixture of p -aminophenol, o -aminophenol and m -aminophenol, which achieved the detection limits of 0.24 μM, 0.41 μM and 0.42 μM, respectively. Finally, the successful detection of antibiotics demonstrated the potential of this dual-channel microchip system for clinical application.
ISSN:1759-9660
1759-9679
DOI:10.1039/C7AY01008G