Loading…

A novel label-free fluorescent molecular beacon for the detection of 3′–5′ exonuclease enzymatic activity using DNA-templated copper nanoclusters

The 3′–5′ exonuclease plays key roles in maintaining genome stability; therefore, the detection of 3′–5′ exonuclease activity is very important for disease diagnosis and drug development. In this study, we present a novel highly sensitive and label-free fluorescent assay to detect the activity of ex...

Full description

Saved in:
Bibliographic Details
Published in:New journal of chemistry 2017, Vol.41 (18), p.9718-9723
Main Authors: Ge, Jia, Dong, Zhen-Zhen, Bai, Dong-Mei, Zhang, Lin, Hu, Ya-Lei, Ji, Dan-Yang, Li, Zhao-Hui
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The 3′–5′ exonuclease plays key roles in maintaining genome stability; therefore, the detection of 3′–5′ exonuclease activity is very important for disease diagnosis and drug development. In this study, we present a novel highly sensitive and label-free fluorescent assay to detect the activity of exonuclease III (Exo III) using molecular beacon DNA with a polythymine (T) loop as a template for the formation of fluorescent copper nanoparticles (CuNPs). Upon the addition of Exo III, the molecular beacon probe was digested into mono- or short oligonucleotides by the exonucleases and failed to form fluorescent CuNPs. As a result, the fluorescence intensity of CuNPs decreased. This method exhibited a very low detection limit of 0.02 U mL −1 for Exo III. The designed molecular beacon probe can be a potential label-free strategy for the detection of other nuclease activity.
ISSN:1144-0546
1369-9261
DOI:10.1039/C7NJ01761H