Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots

The development of an effective method for imaging of lysosomal hypochlorous acid (HClO) is of great significance to better understand the roles of HClO-related diseases. Herein, we report on the fabrication of fluorescence resonance energy transfer (FRET)-based fluorescent polymer dots ( FPD ) for...

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Bibliographic Details
Published in:Polymer chemistry 2017-10, Vol.8 (37), p.5795-5802
Main Authors: Wang, Hong, Zhang, Peisheng, Hong, Yongxiang, Zhao, Bin, Yi, Pinggui, Chen, Jian
Format: Article
Language:English
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Summary:The development of an effective method for imaging of lysosomal hypochlorous acid (HClO) is of great significance to better understand the roles of HClO-related diseases. Herein, we report on the fabrication of fluorescence resonance energy transfer (FRET)-based fluorescent polymer dots ( FPD ) for the ratiometric imaging of lysosomal HClO in living cells via a simple co-precipitation method. This FRET-based construction strategy takes the advantage of flexible design by incorporating target-inert fluorogen (donor) and target-sensitive fluorogen (acceptor) into the nanoparticle to avoid leakage and photobleaching problems. The as-prepared FPD exhibits good water dispersibility, fast response (15 weeks). Moreover, the resultant FPD with good membrane permeability could mainly locate in the lysosomes and act as a probe for the ratiometric imaging of lysosomal HClO in living HeLa cells.
ISSN:1759-9954
1759-9962
DOI:10.1039/C7PY01289F