Promoting early neovascularization of SIS-repaired abdominal wall by controlled release of bioactive VEGF

Insufficient early neovascularization post-operation is thought to be the main reason of surgical recurrence of porcine small intestinal submucosa (SIS)-repaired abdominal wall defects. The controlled release of exogenous angiogenic growth factors (GFs) from biocompatible carriers is a possible way...

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Bibliographic Details
Published in:RSC advances 2018-01, Vol.8 (9), p.4548-456
Main Authors: Tang, Rui, Wang, Xin, Zhang, Hanying, Liang, Xi, Feng, Xueyi, Zhu, Xiaoqiang, Lu, Xinwu, Wu, Fei, Liu, Zhengni
Format: Article
Language:English
Subjects:
Biocompatibility
Biological activity
Chemistry
Controlled release
Electrospinning
Glycolic acid
Growth factors
In vivo methods and tests
Phase separation
Scaffolds
Vascular endothelial growth factor
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Summary:Insufficient early neovascularization post-operation is thought to be the main reason of surgical recurrence of porcine small intestinal submucosa (SIS)-repaired abdominal wall defects. The controlled release of exogenous angiogenic growth factors (GFs) from biocompatible carriers is a possible way to solve this problem. In the present study, dextran nanoparticles (DNPs) loaded with vascular endothelial growth factor 165 (VEGF 165 ) were pre-formulated by dual-aqueous phase separation method and then electrospun into the poly(lactic- co -glycolic acid) (PLGA) polymer fibers. The aim of this material is to release VEGF in a sustained manner with the degradation of PLGA and maintain its bioactivity concurrently. The prepared VEGF/DNPs-PLGA membrane was sandwiched by dual-layer SIS to construct a SIS-DNPs/VEGF-PLGA-SIS (SVDPS) composite scaffold. The in vitro study showed that the VEGF/DNPs-PLGA obtained higher VEGF encapsulation efficiency as well as better release property and bioactivity than the emulsion electrospun VEGF-PLGA and PLGA fibrous membranes by ELISA and HUVEC proliferation. The in vivo study showed that the SVDPS composite scaffold promoted significantly higher early therapeutic neovascularization within 2 weeks post-surgery than SIS-VEGF-PLGA-SIS (SVPS) and SIS-PLGA-SIS (SPS) by immunohistochemical and immunoblotting examination. Insufficient early neovascularization post-operation is thought to be the main reason of surgical recurrence of porcine small intestinal submucosa (SIS)-repaired abdominal wall defects.
ISSN:2046-2069
2046-2069
DOI:10.1039/c7ra11954b