Rapid "turn-on" photoluminescence detection of bisulfite in wines and living cells with a formyl bearing bis-cyclometalated Ir() complex

• Published in: Analyst (London) 2018-07, Vol.143 (15), p.367-3676
• Main Authors: Gao, Hongfang, Qi, Hetong, Peng, Yijin, Qi, Honglan, Zhang, Chengxiao
• Format: Article
• Language: English
• Subjects: Acetic acid; Buffer solutions; Cells (biology); Fluorescent Dyes; Humans; Iridium; Ligands; Luminescent Measurements; MCF-7 Cells; Medical imaging; Phenanthrolines; Photoluminescence; Pyridines; Sodium acetate; Sulfites - analysis; Wine - analysis; Wines
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/c8an00640g

A new photoluminescence (PL) probe based on a formyl bearing bis-cyclometalated Ir( iii ) complex, [Ir(ppy) 2 phen-CHO] + PF 6 − ( 1 ), is synthesized and applied to the selective detection of a bisulfite anion (HSO 3 − ). Probe 1 is prepared using 2-phenylpyridine (ppy) as the C^N main ligand and 1,10-phenanthroline-5-carboxaldehyde (phen-CHO) as the N^N ancillary ligand. Probe 1 displayed excellent selective PL enhancement in response to HSO 3 − in acetic acid-sodium acetate buffer solution (pH = 5.0). The increase of PL signal is directly proportional to the concentration of HSO 3 − in the range of 2 μM to 45 μM with a detection limit of 0.9 μM using 50 μM probe 1 and in the range of 0.5 μM to 6 μM with a detection limit of 0.3 μM using 10 μM probe 1 . More importantly, probe 1 can respond to HSO 3 − rapidly within 40 s. Furthermore, probe 1 was successfully applied to detect HSO 3 − in real white wines and the bioimaging of HSO 3 − in living cells. The superior properties of probe 1 make it of great potential use for studying the effects of HSO 3 − in other biosystems. A new photoluminescence (PL) probe based on a formyl bearing bis-cyclometalated Ir( iii ) complex, [Ir(ppy) 2 phen-CHO] + PF 6 − ( 1 ), is synthesized and applied to the selective detection of a bisulfite anion (HSO 3 − ).