A continuous droplet electroporation system for high throughput processing

A continuous droplet electroporation (EP) system capable of handling a billion cells has been proposed and demonstrated using a proof-of-concept prototype design. Numerical simulations were conducted to design the new system and to compare the continuous droplet EP system with the previous single dr...

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Bibliographic Details
Published in:Analyst (London) 2018-11, Vol.143 (23), p.5785-5791
Main Authors: Yoo, Byeong Sun, Im, Do Jin, Ahn, Myung Mo, Park, Sung Jin, Kim, Yeong Hun, Um, Tae Woong, Kang, In Seok
Format: Article
Language:English
Subjects:
Chlamydomonas reinhardtii
Computer simulation
DNA - genetics
Droplets
Electroporation
Electroporation - instrumentation
Flow characteristics
Flow velocity
Fluorescence
Gene Transfer Techniques
Green Fluorescent Proteins - genetics
Plasmids - genetics
Proteins
Transgenes - genetics
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Summary:A continuous droplet electroporation (EP) system capable of handling a billion cells has been proposed and demonstrated using a proof-of-concept prototype design. Numerical simulations were conducted to design the new system and to compare the continuous droplet EP system with the previous single droplet EP system. Through parametric studies on the applied voltage and flow rate, a much higher cyan fluorescent protein transgene expression efficiency (38.8 ± 8.9%) was accomplished over that of the previous single droplet EP system. A parallel continuous droplet EP system is also demonstrated by introducing additional electrode pairs into the continuous droplet EP system to achieve ultrahigh throughput. Finally, the significance and meaning of the present work and future development direction have been discussed. A continuous droplet electroporation (EP) system capable of handling a billion cells has been proposed and demonstrated using a proof-of-concept prototype design.
ISSN:0003-2654
1364-5528
DOI:10.1039/c8an01259h