Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes

c-Jun N-terminal kinases (JNKs) are involved in critical cellular functions. Herein, small-molecule JNK-targeting probes are reported based on a covalent inhibitor. Together with newly developed two-photon fluorescence Turn-ON reporters and chemoproteomic studies, we showed that some probes may be s...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2019-01, Vol.55 (8), p.192-195
Main Authors: Qian, Linghui, Pan, Sijun, Lee, Jun-Seok, Ge, Jingyan, Li, Lin, Yao, Shao Q
Format: Article
Language:English
Subjects:
Fluorescence
Inhibitors
Kinases
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Summary:c-Jun N-terminal kinases (JNKs) are involved in critical cellular functions. Herein, small-molecule JNK-targeting probes are reported based on a covalent inhibitor. Together with newly developed two-photon fluorescence Turn-ON reporters and chemoproteomic studies, we showed that some probes may be suitable for live-cell imaging and profiling of JNKs. Bioorthogonal probes which feature covalent labelling, and the corresponding two-photon fluorogenic reporters, are used to profile and image cellular JNKs.
ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc09558b