Magnetic surface molecularly imprinted poly(3-aminophenylboronic acid) for selective capture and determination of diethylstilbestrol

• Published in: RSC advances 2018-04, Vol.8 (24), p.13129-13141
• Main Authors: Zhao, Wen-Rui, Kang, Tian-Fang, Lu, Li-Ping, Cheng, Shui-Yuan
• Format: Article
• Language: English
• Subjects: Chemistry; Environmental protection; High performance liquid chromatography; Imprinted polymers; Infants; Iron oxides; Isotherms; Magnetic cores; Molecular imprinting; Nanoparticles; Recognition; Reproductive system; Silicon dioxide; Synthesis
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/c8ra01250d

Diethylstilbestrol (DES) is considered a representative example of an exogenous endocrine disrupting compound (EDC). It can retard development in infants, lead to serious metabolic regulation disorders, and even result in distortion and cancer in the reproductive system. Therefore, achieving rapid and accurate analysis of trace amounts of DES in complex environments is of great importance to human health and for environmental protection. Novel magnetic molecularly imprinted polymers (MIPs) with excellent molecular recognition ability and super water-compatibility were developed for the selective capture of DES in water samples. Fe 3 O 4 @SiO 2 magnetic nanoparticles (NPs) were synthesized and used as support cores. Molecularly imprinted poly(3-aminophenylboronic acid) (poly(APBA)), synthesized on magnetic cores based on a surface-imprinting strategy, can preferentially bind DES molecules in water samples. The magnetic core-shell MIPs (denoted as Fe 3 O 4 @SiO 2 @APBA/MIPs) exhibited high binding capacity and favorable recognition specificity for DES in water. The adsorption kinetics and experimental isotherm data of DES on magnetic MIPs can be well described by the pseudo-second-order kinetic model and the Langmuir isotherm, respectively. The imprinted nanoparticles were subjected to magnetic solid-phase extraction (MSPE) of DES from water samples. The DES content in the samples was determined by high-performance liquid chromatography (HPLC). The peak area increased linearly with increasing DES concentration over the range 0.08-150 μg L −1 , with a detection limit of 0.03 μg L −1 . The recoveries for spiked lake water samples were in the range 97.1-103.2%, with relative standard deviation (RSD) of 2.8-4.3% ( n = 6). Imprinted poly(APBA) nanoshell on Fe 3 O 4 @SiO 2 surface was first synthesized and used for MSPE of diethylstilbestrol followed by HPLC determination.