Core-independent approach for polymer brush-functionalised nanomaterials with a fluorescent tag for RNA delivery

Here we describe a core-independent approach enabling the grafting of polymer brushes from the surface of nanomaterials and microparticles for oligonucleotide delivery. This method is based on the adsorption of a polyelectrolyte macroinitiator (MI) combined with a fluorescent conjugated polymer for...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2019-12, Vol.55 (94), p.14166-14169
Main Authors: Li, Danyang, Wu, Linke, Qu, Fengjin, Ribadeneyra, Maria Crespo, Tu, Guoli, Gautrot, Julien
Format: Article
Language:English
Subjects:
Brushes
Cell Line, Tumor
Cell membranes
Cell Survival - drug effects
Drug Carriers - chemistry
Fluorescence
Humans
Initiators
Microparticles
Nanomaterials
Nanostructures - chemistry
Organic chemistry
Particle Size
Polyelectrolytes
Polyelectrolytes - chemistry
Polymers
Polymers - chemistry
Polymers - pharmacology
RNA - administration & dosage
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Summary:Here we describe a core-independent approach enabling the grafting of polymer brushes from the surface of nanomaterials and microparticles for oligonucleotide delivery. This method is based on the adsorption of a polyelectrolyte macroinitiator (MI) combined with a fluorescent conjugated polymer for efficient and stable labelling. This allows dense brushes to be generated, with growth kinetics comparable to those observed from mono-functional initiators, for the imaging of nanomaterial cellular localisation and uptake. We also study the impact of brush chemistry on interactions with cell membranes and on transfection efficiency. The method we report offers a unique freedom of design of the core size and shape as well as surface chemistry, whilst enabling tagging, for the study of transfection processes or theragnostic applications. A core-independent approach for the design of polymer brush-functionalised nanomaterials with a fluorescent tag for RNA delivery.
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc05790k