9t18:1 and 11t18:1 activate the MAPK pathway to regulate the expression of PLA2 and cause inflammation in HUVECs

trans fatty acids (TFAs) have been reported to promote vascular diseases mainly by promoting apoptosis and inflammation of vascular endothelial cells. However, it has been reported in recent years that elaidic acid (9t18:1) and vaccenic acid (11t18:1) may have different effects on vascular health. T...

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Bibliographic Details
Published in:Food & function 2020-01, Vol.11 (1), p.649-661
Main Authors: Hu, Sheng-Ben, Zou, Qian, Lv, Xin, Zhou, Ruo-Lin, Niu, Xian, Weng, Chen, Chen, Fang, Fan, Ya-Wei, Deng, Ze-Yuan, Li, Jing
Format: Article
Language:English
Subjects:
Apoptosis
Cell viability
Composition effects
Cyclooxygenase-2
Cytokines
Endothelial cells
Fatty acids
Inflammation
MAP kinase
Phospholipase
Phospholipase A2
Phospholipid composition
Phospholipids
Prostaglandin E2
Trans fatty acids
Umbilical vein
Vascular diseases
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Summary:trans fatty acids (TFAs) have been reported to promote vascular diseases mainly by promoting apoptosis and inflammation of vascular endothelial cells. However, it has been reported in recent years that elaidic acid (9t18:1) and vaccenic acid (11t18:1) may have different effects on vascular health. This study investigated the effects of 9t18:1 and 11t18:1 on human umbilical vein endothelial cell (HUVEC) function and the possible mechanism of inflammation by analyzing the changes in the phospholipid composition and the relationship between phospholipase A2 (PLA2) and MAPK pathway. Here we found that the effect of 11t18:1 on cell viability, membrane damage and cellular inflammation was significantly lower than that of 9t18:1 ( p < 0.05). And 9t18:1 and 11t18:1 had different effects on phospholipid composition. Both 9t18:1 and 11t18:1 significantly increased the protein expression of PLA2. Moreover, the MAPK pathway regulated the expression of PLA2, inflammatory cytokines and cyclooxygenase-2 (COX-2) and the secretion of prostaglandin E2 (PGE2) in HUVECs induced by 9t18:1 and 11t18:1. In conclusion, 9t18:1 and 11t18:1 activated the MAPK pathway which regulated the expression of PLA2 to cause inflammation in HUVECs. trans fatty acids (TFAs) have been reported to promote vascular diseases mainly by promoting apoptosis and inflammation of vascular endothelial cells.
ISSN:2042-6496
2042-650X
DOI:10.1039/c9fo01982k