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A ratiometric bilirubin sensor based on a fluorescent gold nanocluster film with dual emissions
Bilirubin originates from hemoglobin metabolism and is an important biomarker for liver function. A ratiometric film sensor based on gold nanoclusters (AuNCs) was fabricated for highly sensitive determination of free bilirubin (fBR). Using bovine serum albumin (BSA) as a template, AuNCs that can emi...
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Published in: | Analytical methods 2020-12, Vol.12 (47), p.5691-5698 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Bilirubin originates from hemoglobin metabolism and is an important biomarker for liver function. A ratiometric film sensor based on gold nanoclusters (AuNCs) was fabricated for highly sensitive determination of free bilirubin (fBR). Using bovine serum albumin (BSA) as a template, AuNCs that can emit blue and red fluorescence were prepared by the hydrothermal method at different pH values. Two kinds of AuNCs were incorporated into a single film by the layer-by-layer assembly (LBL) technique. The obtained thin-film showed dual fluorescence peaks excited at 372 nm, corresponding to the blue (443 nm) and red (622 nm) emissions of AuNCs respectively. When fBR interacted with the film, both fluorescence peaks were quenched at different degrees. A ratiometric method for fBR detection was established based on the fluorescence intensity ratio of the two emissions. The linear calibration curve for fBR lay in the concentration range of 0.01-2.00 μmol L
−1
with a detection limit of 8.90 ± 0.34 nmol L
−1
(S/N = 3). The film sensor showed a quick and sensitive response to fBR and could detect fBR in real samples with satisfactory results.
Bilirubin originates from hemoglobin metabolism and is an important biomarker for liver function. |
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ISSN: | 1759-9660 1759-9679 |
DOI: | 10.1039/d0ay01781g |