A visible and near-infrared dual-fluorescent probe for discrimination between Cys/Hcy and GSH and its application in bioimaging

Biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play important roles in various physiological and pathological processes, and due to their similar structures and reaction activities, it is still challenging to simultaneously discriminate between GSH and Cys/Hcy in vivo . H...

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Bibliographic Details
Published in:Biomaterials science 2020-11, Vol.8 (21), p.5994-63
Main Authors: Li, Ruixi, Kassaye, Habtamu, Pan, Yanping, Shen, Yuanzhi, Li, Weiqing, Cheng, Yuru, Guo, Jingxuan, Xu, Yue, Yin, Hongping, Yuan, Zhenwei
Format: Article
Language:English
Subjects:
Animals
Biological properties
Cysteine
Diagnostic Imaging
Emission
Fluorescent Dyes
Fluorescent indicators
Glutathione
HeLa Cells
Homocysteine
Humans
Medical imaging
Mice
Microscopy, Fluorescence
Near infrared radiation
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Summary:Biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play important roles in various physiological and pathological processes, and due to their similar structures and reaction activities, it is still challenging to simultaneously discriminate between GSH and Cys/Hcy in vivo . Hence, a novel fluorescent probe for simultaneously discriminating GSH and Cys/Hcy in biological samples is highly desirable. Herein, we presented two enhanced fluorescent probes (Cy1 and Cy2) with doubly-activated dual emission for sensitive and discriminative detection of Cys/Hcy and GSH. The probes were constructed with IR-780 and NBD linked via an ether bond, which responds to GSH with near infrared (NIR) emission at 810 nm ( λ ex = 720 nm) and Cys/Hcy with visible green emission at 550 nm ( λ ex = 470 nm). The probe Cy2 is operable in human serum samples, thus holding promise for its diagnosis-related application. Notably, the results of fluorescence microscopy imaging indicated that Cy2 is suitable for visualization of exogenous and endogenous biothiols in living cells. Furthermore, desirable results were obtained when the probe Cy2 was applied for bioimaging in tumor-bearing mice and acute liver injury (ALI) mice models, which revealed encouraging clinical values of this probe. The probe Cy2 showed high sensitivity and excellent selectivity with a distinct fluorescence off-on response to GSH with NIR emission and Cys/Hcy with green emission, respectively.
ISSN:2047-4830
2047-4849
DOI:10.1039/d0bm01237h