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Facile synthesis of Cu-CuFe 2 O 4 nanozymes for sensitive assay of H 2 O 2 and GSH
Artificial enzymes have drawn substantial research interest from the scientific community due to their advantages over natural enzymes. However, majority of artificial enzymes exhibit low affinity towards H 2 O 2 , which means that a high H 2 O 2 concentration is needed for the oxidation of a substr...
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| Published in: | Dalton transactions : an international journal of inorganic chemistry 2020-09, Vol.49 (36), p.12780-12792 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
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| Summary: | Artificial enzymes have drawn substantial research interest from the scientific community due to their advantages over natural enzymes. However, majority of artificial enzymes exhibit low affinity towards H
2
O
2
, which means that a high H
2
O
2
concentration is needed for the oxidation of a substrate such as 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-colored oxTMB. With this concern, Cu-CuFe
2
O
4
was facilely synthesized, wherein, Cu
0
accelerates the redox capacity of Cu-CuFe
2
O
4
as well as the electron transfer between CuFe
2
O
4
and H
2
O
2
. These materials induce excellent activity as a peroxidase. Cu-CuFe
2
O
4
shows high affinity towards H
2
O
2
with lower Michaelis–Menten constant (
K
m
) than the reported values for ferrites and Horseradish enzyme (HRP). Moreover, it took only 5 min to detect hydrogen peroxide (H
2
O
2
) and glutathione (GSH) through a colorimetric assay using Cu-CuFe
2
O
4
. Compared with CuFe
2
O
4
, the limit of detection (LOD) is about 90-fold lower for H
2
O
2
using Cu-CuFe
2
O
4
. In addition, Cu-CuFe
2
O
4
shows high stability as a nanozyme. Thus, the mechanism of the peroxidase-like nanozyme Cu-CuFe
2
O
4
is proposed. |
|---|---|
| ISSN: | 1477-9226 1477-9234 |
| DOI: | 10.1039/D0DT02395G |