A novel split mode TFBAR device for quantitative measurements of prostate specific antigen in a small sample of whole blood

Easy monitoring of prostate specific antigen (PSA) directly from blood samples would present a significant improvement as compared to conventional diagnostic methods. In this work, a split mode thin film bulk acoustic resonator (TFBAR) device was employed for the first time for label-free measuremen...

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Bibliographic Details
Published in:Nanoscale 2020-05, Vol.12 (17), p.9647-9652
Main Authors: Wajs, Ewelina, Rughoobur, Girish, Burling, Keith, George, Anne, Flewitt, Andrew J, Gnanapragasam, Vincent J
Format: Article
Language:English
Subjects:
Acoustics
Antibodies
Antigens
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Blood
Bulk acoustic wave devices
Diagnostic systems
Humans
Immunoassay
Limit of Detection
Male
Monitoring
Optimization
Physicians
Point-of-Care Testing
Pretreatment
Prostate
Prostate-Specific Antigen - blood
Signal reconstruction
Telemedicine
Thin films
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Summary:Easy monitoring of prostate specific antigen (PSA) directly from blood samples would present a significant improvement as compared to conventional diagnostic methods. In this work, a split mode thin film bulk acoustic resonator (TFBAR) device was employed for the first time for label-free measurements of PSA concentrations in the whole blood and without sample pre-treatment. The surface of the sensor was covalently modified with anti-PSA antibodies and demonstrated a very high sensitivity of 101 kHz mL ng −1 and low limit of detection (LOD) of 0.34 ng mL −1 in model spiked solutions. It has previously been widely believed that significant pre-processing of blood samples would be required for TFBAR biosensors. Importantly, this work demonstrates that this is not the case, and TFBAR technology provides a cost-effective means for point-of-care (POC) diagnostics and monitoring of PSA in hospitals and in doctors' offices. Additionally, the accuracy of the developed biosensor, with respect to a commercial auto analyser (Beckman Coulter Access), was evaluated to analyse clinical samples, giving well-matched results between the two methods, thus showing a practical application in quantitative monitoring of PSA levels in the whole blood with very good signal recovery. TFBAR technology demonstrates a cost-effective means for point-of-care diagnostics and monitoring of PSA.
ISSN:2040-3364
2040-3372
DOI:10.1039/d0nr00416b