An effective polymeric nanocarrier that allows for active targeting and selective drug delivery in cell coculture systems

In this manuscript, we report the development of a versatile, robust, and stable targeting nanocarrier for active delivery. This nanocarrier is based on bifunctionalized polymeric nanoparticles conjugated to a monoclonal antibody that allows for active targeting of either (i) a fluorophore for track...

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Bibliographic Details
Published in:Nanoscale 2021-02, Vol.13 (6), p.35-3511
Main Authors: Cano-Cortes, Maria Victoria, Altea-Manzano, Patricia, Laz-Ruiz, Jose Antonio, Unciti-Broceta, Juan Diego, Lopez-Delgado, Francisco Javier, Espejo-Roman, Jose Manuel, Diaz-Mochon, Juan Jose, Sanchez-Martin, Rosario M
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Coculture Techniques
Drug Carriers
Drug Delivery Systems
Growth factors
Monoclonal antibodies
Nanoparticles
Nanotechnology devices
Polymers
Receptors
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Summary:In this manuscript, we report the development of a versatile, robust, and stable targeting nanocarrier for active delivery. This nanocarrier is based on bifunctionalized polymeric nanoparticles conjugated to a monoclonal antibody that allows for active targeting of either (i) a fluorophore for tracking or (ii) a drug for monitoring specific cell responses. This nanodevice can efficiently discriminate between cells in coculture based on the expression levels of cell surface receptors. As a proof of concept, we have demonstrated efficient delivery using a broadly established cell surface receptor as the target, the epidermal growth factor receptor (EGFR), which is overexpressed in several types of cancers. Additionally, a second validation of this nanodevice was successfully carried out using another cell surface receptor as the target, the cluster of differentiation 147 (CD147). Our results suggest that this versatile nanocarrier can be expanded to other cell receptors and bioactive cargoes, offering remarkable discrimination efficiency between cells with different expression levels of a specific marker. This work supports the ability of nanoplatforms to boost and improve the progress towards personalized medicine. A versatile and robust approach for active targeted delivery based on the use of antibody-decorated nanoparticles offering remarkable discrimination efficiency between cocultured cells with different expression levels of a specific marker has been developed.
ISSN:2040-3364
2040-3372
DOI:10.1039/d0nr07145e