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Decellularised extracellular matrix decorated PCL PolyHIPE scaffolds for enhanced cellular activity, integration and angiogenesis
Wound healing involves a complex series of events where cell-cell and cell-extracellular matrix (ECM) interactions play a key role. Wounding can be simple, such as the loss of the epithelial integrity, or deeper and more complex, reaching to subcutaneous tissues, including blood vessels, muscles and...
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Published in: | Biomaterials science 2021-10, Vol.9 (21), p.7297-731 |
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creator | Dikici, Serkan Aldemir Dikici, Betül MacNeil, Sheila Claeyssens, Frederik |
description | Wound healing involves a complex series of events where cell-cell and cell-extracellular matrix (ECM) interactions play a key role. Wounding can be simple, such as the loss of the epithelial integrity, or deeper and more complex, reaching to subcutaneous tissues, including blood vessels, muscles and nerves. Rapid neovascularisation of the wounded area is crucial for wound healing as it has a key role in supplying oxygen and nutrients during the highly demanding proliferative phase and transmigration of inflammatory cells to the wound area. One approach to circumvent delayed neovascularisation is the exogenous use of pro-angiogenic factors, which is expensive, highly dose-dependent, and the delivery of them requires a very well-controlled system to avoid leaky, highly permeable and haemorrhagic blood vessel formation. In this study, we decorated polycaprolactone (PCL)-based polymerised high internal phase emulsion (PolyHIPE) scaffolds with fibroblast-derived ECM to assess fibroblast, endothelial cell and keratinocyte activity
in vitro
and angiogenesis in
ex ovo
chick chorioallantoic membrane (CAM) assays. Our results showed that the inclusion of ECM in the scaffolds increased the metabolic activity of three types of cells that play a key role in wound healing and stimulated angiogenesis in
ex ovo
CAM assays over 7 days. Herein, we demonstrated that fibroblast-ECM functionalised PCL PolyHIPE scaffolds appear to have great potential to be used as an active wound dressing to promote angiogenesis and wound healing.
Decellularisation of
in vitro
generated extracellular matrix (ECM) provides an effective way to stimulate angiogenesis and wound healing. |
doi_str_mv | 10.1039/d1bm01262b |
format | article |
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in vitro
and angiogenesis in
ex ovo
chick chorioallantoic membrane (CAM) assays. Our results showed that the inclusion of ECM in the scaffolds increased the metabolic activity of three types of cells that play a key role in wound healing and stimulated angiogenesis in
ex ovo
CAM assays over 7 days. Herein, we demonstrated that fibroblast-ECM functionalised PCL PolyHIPE scaffolds appear to have great potential to be used as an active wound dressing to promote angiogenesis and wound healing.
Decellularisation of
in vitro
generated extracellular matrix (ECM) provides an effective way to stimulate angiogenesis and wound healing.</description><identifier>ISSN: 2047-4830</identifier><identifier>EISSN: 2047-4849</identifier><identifier>DOI: 10.1039/d1bm01262b</identifier><identifier>PMID: 34617526</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Angiogenesis ; Blood vessels ; Chemistry ; Emulsion polymerization ; Endothelial cells ; Extracellular matrix ; Fibroblasts ; Muscles ; Nerves ; Nutrients ; Polycaprolactone ; Scaffolds ; Wound healing</subject><ispartof>Biomaterials science, 2021-10, Vol.9 (21), p.7297-731</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><rights>This journal is © The Royal Society of Chemistry 2021 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-b3aa81830eb3edf3a582503b8a0e1a447bfb053916c56207996f773d4b3237593</citedby><cites>FETCH-LOGICAL-c446t-b3aa81830eb3edf3a582503b8a0e1a447bfb053916c56207996f773d4b3237593</cites><orcidid>0000-0002-5516-469X ; 0000-0001-9933-5254</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids></links><search><creatorcontrib>Dikici, Serkan</creatorcontrib><creatorcontrib>Aldemir Dikici, Betül</creatorcontrib><creatorcontrib>MacNeil, Sheila</creatorcontrib><creatorcontrib>Claeyssens, Frederik</creatorcontrib><title>Decellularised extracellular matrix decorated PCL PolyHIPE scaffolds for enhanced cellular activity, integration and angiogenesis</title><title>Biomaterials science</title><description>Wound healing involves a complex series of events where cell-cell and cell-extracellular matrix (ECM) interactions play a key role. Wounding can be simple, such as the loss of the epithelial integrity, or deeper and more complex, reaching to subcutaneous tissues, including blood vessels, muscles and nerves. Rapid neovascularisation of the wounded area is crucial for wound healing as it has a key role in supplying oxygen and nutrients during the highly demanding proliferative phase and transmigration of inflammatory cells to the wound area. One approach to circumvent delayed neovascularisation is the exogenous use of pro-angiogenic factors, which is expensive, highly dose-dependent, and the delivery of them requires a very well-controlled system to avoid leaky, highly permeable and haemorrhagic blood vessel formation. In this study, we decorated polycaprolactone (PCL)-based polymerised high internal phase emulsion (PolyHIPE) scaffolds with fibroblast-derived ECM to assess fibroblast, endothelial cell and keratinocyte activity
in vitro
and angiogenesis in
ex ovo
chick chorioallantoic membrane (CAM) assays. Our results showed that the inclusion of ECM in the scaffolds increased the metabolic activity of three types of cells that play a key role in wound healing and stimulated angiogenesis in
ex ovo
CAM assays over 7 days. Herein, we demonstrated that fibroblast-ECM functionalised PCL PolyHIPE scaffolds appear to have great potential to be used as an active wound dressing to promote angiogenesis and wound healing.
Decellularisation of
in vitro
generated extracellular matrix (ECM) provides an effective way to stimulate angiogenesis and wound healing.</description><subject>Angiogenesis</subject><subject>Blood vessels</subject><subject>Chemistry</subject><subject>Emulsion polymerization</subject><subject>Endothelial cells</subject><subject>Extracellular matrix</subject><subject>Fibroblasts</subject><subject>Muscles</subject><subject>Nerves</subject><subject>Nutrients</subject><subject>Polycaprolactone</subject><subject>Scaffolds</subject><subject>Wound healing</subject><issn>2047-4830</issn><issn>2047-4849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkc9rFDEUx4NYbFl78S4MeBFxbX5n5iK029YWVtyDnkOSebNNmUlqMlO6R_9zs9260gYeCe998uV9-SL0juAvBLPmpCV2wIRKal-hI4q5mvOaN6_3b4YP0XHOt7gcpRosyRt0yLgkSlB5hP6cg4O-n3qTfIa2gocxmX-dajBj8g9VCy4mM5bxarGsVrHfXF2vLqrsTNfFvs1VF1MF4cYEV5j9b-NGf-_HzefKhxHWRcHHUJnQllr7uIYA2ee36KAzfYbjp3uGfl1e_FxczZc_vl0vTpdzx7kc55YZU5NiByyDtmNG1FRgZmuDgRjOle0sFqwh0glJsWoa2SnFWm4ZZUo0bIa-7nTvJjtA6yAUp72-S34waaOj8fr5JPgbvY73uhZcMVoXgY9PAin-niCPevB5a9YEiFPWVNQYU7zdaoY-vEBv45RCsbelRFOSw7JQn3aUSzHnBN1-GYL1Nlx9Ts6-P4Z7VuD3Ozhlt-f-h8_-Anx2oPI</recordid><startdate>20211026</startdate><enddate>20211026</enddate><creator>Dikici, Serkan</creator><creator>Aldemir Dikici, Betül</creator><creator>MacNeil, Sheila</creator><creator>Claeyssens, Frederik</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5516-469X</orcidid><orcidid>https://orcid.org/0000-0001-9933-5254</orcidid></search><sort><creationdate>20211026</creationdate><title>Decellularised extracellular matrix decorated PCL PolyHIPE scaffolds for enhanced cellular activity, integration and angiogenesis</title><author>Dikici, Serkan ; Aldemir Dikici, Betül ; MacNeil, Sheila ; Claeyssens, Frederik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-b3aa81830eb3edf3a582503b8a0e1a447bfb053916c56207996f773d4b3237593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Angiogenesis</topic><topic>Blood vessels</topic><topic>Chemistry</topic><topic>Emulsion polymerization</topic><topic>Endothelial cells</topic><topic>Extracellular matrix</topic><topic>Fibroblasts</topic><topic>Muscles</topic><topic>Nerves</topic><topic>Nutrients</topic><topic>Polycaprolactone</topic><topic>Scaffolds</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dikici, Serkan</creatorcontrib><creatorcontrib>Aldemir Dikici, Betül</creatorcontrib><creatorcontrib>MacNeil, Sheila</creatorcontrib><creatorcontrib>Claeyssens, Frederik</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomaterials science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dikici, Serkan</au><au>Aldemir Dikici, Betül</au><au>MacNeil, Sheila</au><au>Claeyssens, Frederik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decellularised extracellular matrix decorated PCL PolyHIPE scaffolds for enhanced cellular activity, integration and angiogenesis</atitle><jtitle>Biomaterials science</jtitle><date>2021-10-26</date><risdate>2021</risdate><volume>9</volume><issue>21</issue><spage>7297</spage><epage>731</epage><pages>7297-731</pages><issn>2047-4830</issn><eissn>2047-4849</eissn><abstract>Wound healing involves a complex series of events where cell-cell and cell-extracellular matrix (ECM) interactions play a key role. Wounding can be simple, such as the loss of the epithelial integrity, or deeper and more complex, reaching to subcutaneous tissues, including blood vessels, muscles and nerves. Rapid neovascularisation of the wounded area is crucial for wound healing as it has a key role in supplying oxygen and nutrients during the highly demanding proliferative phase and transmigration of inflammatory cells to the wound area. One approach to circumvent delayed neovascularisation is the exogenous use of pro-angiogenic factors, which is expensive, highly dose-dependent, and the delivery of them requires a very well-controlled system to avoid leaky, highly permeable and haemorrhagic blood vessel formation. In this study, we decorated polycaprolactone (PCL)-based polymerised high internal phase emulsion (PolyHIPE) scaffolds with fibroblast-derived ECM to assess fibroblast, endothelial cell and keratinocyte activity
in vitro
and angiogenesis in
ex ovo
chick chorioallantoic membrane (CAM) assays. Our results showed that the inclusion of ECM in the scaffolds increased the metabolic activity of three types of cells that play a key role in wound healing and stimulated angiogenesis in
ex ovo
CAM assays over 7 days. Herein, we demonstrated that fibroblast-ECM functionalised PCL PolyHIPE scaffolds appear to have great potential to be used as an active wound dressing to promote angiogenesis and wound healing.
Decellularisation of
in vitro
generated extracellular matrix (ECM) provides an effective way to stimulate angiogenesis and wound healing.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>34617526</pmid><doi>10.1039/d1bm01262b</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-5516-469X</orcidid><orcidid>https://orcid.org/0000-0001-9933-5254</orcidid><oa>free_for_read</oa></addata></record> |
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source | Royal Society of Chemistry Journals |
subjects | Angiogenesis Blood vessels Chemistry Emulsion polymerization Endothelial cells Extracellular matrix Fibroblasts Muscles Nerves Nutrients Polycaprolactone Scaffolds Wound healing |
title | Decellularised extracellular matrix decorated PCL PolyHIPE scaffolds for enhanced cellular activity, integration and angiogenesis |
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