Development of 1,8-naphthalimide dyes for rapid imaging of subcellular compartments in plants

Here, we report the installation of 1,8-naphthalimide dyes in live cell imaging of plants. We developed a series of 1,8-naphthalimide-based probes that illuminate different subcellular compartments by altering their spectral characteristics. Simple infiltration of the probes into leaves rapidly visu...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2022-02, Vol.58 (11), p.1685-1688
Main Authors: Kusano, Shuhei, Nakamura, Sakuya, Izumi, Masanori, Hagihara, Shinya
Format: Article
Language:English
Subjects:
Chloroplasts
Chloroplasts - chemistry
Chloroplasts - metabolism
Compartments
Dyes
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Molecular Structure
Naphthalimides - chemical synthesis
Naphthalimides - chemistry
Optical Imaging
Plant Leaves - chemistry
Vacuoles - chemistry
Vacuoles - metabolism
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Summary:Here, we report the installation of 1,8-naphthalimide dyes in live cell imaging of plants. We developed a series of 1,8-naphthalimide-based probes that illuminate different subcellular compartments by altering their spectral characteristics. Simple infiltration of the probes into leaves rapidly visualized the structure of chloroplasts or the vacuole. We further demonstrated that these probes are applicable to monitor the organelle behaviors in an autophagy pathway. We report the installation of 1,8-naphthalimide dyes in live cell imaging of plants. The structure of the chloroplasts or vacuole was rapidly and clearly visualized by 1,8-naphthalimide dyes.
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/d1cc05798g