Fabrication of a ferritin-casein phosphopeptide-calcium shell-core composite as a novel calcium delivery strategy

Plant ferritin has a natural cage-like nanospace for carrying bioactive ingredients. By taking advantage of the calcium binding ability of casein phosphopeptide (CPP) and the cage-like conformation of plant ferritin, a ferritin-CPP shell-core complex (FC) was fabricated with the reversible self-asse...

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Bibliographic Details
Published in:Food & function 2021-11, Vol.12 (22), p.11378-11386
Main Authors: Zhu, Lei, Shi, Lina, Wang, Qiao-E, Meng, Demei, Zhou, Zhongkai, Yang, Rui
Format: Article
Language:English
Subjects:
Binding
Bioavailability
Cages
Calcium - chemistry
Calcium ions
Calcium phosphates
Casein
Caseins - chemistry
Conformation
Drug Carriers - chemistry
Encapsulation
Fabrication
Ferritin
Ferritins - chemistry
Fluorescence
Fourier analysis
Fourier transforms
Infrared analysis
Ions
Light scattering
Morphology
Nanocomposites - chemistry
Phosphopeptides - chemistry
Photon correlation spectroscopy
Self-assembly
Transmission electron microscopy
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Summary:Plant ferritin has a natural cage-like nanospace for carrying bioactive ingredients. By taking advantage of the calcium binding ability of casein phosphopeptide (CPP) and the cage-like conformation of plant ferritin, a ferritin-CPP shell-core complex (FC) was fabricated with the reversible self-assembly character of ferritin induced by a pH 2.0/7.0 transition strategy. The FC-calcium composite (FCC) was further fabricated by binding of the FC with calcium ions. When the same amount of calcium was loaded, the calcium binding capacity of the FCC was 28.13 ± 1.65%, which was significantly higher than that of ferritin and CPP alone. Fluorescence and Fourier transform infrared analysis indicated that the CPP encapsulation and the calcium binding in the FCC influenced the ferritin structure. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) results showed that the spherical morphology and the 12 nm-diameter size were sustained in the FC and FCC. Moreover, the FCC as a transport carrier could increase the precipitation time of calcium phosphate, and the encapsulated calcium could be released in a more sustained manner as compared with ferritin and CPP under simulated in vitro gastrointestinal conditions. This study presents a novel calcium delivery strategy based on the ferritin cage and CPP, which will improve the applicability of ferritin and CPP and enhance the bioavailability of calcium ions. Fabrication of a ferritin-casein phosphopeptide-calcium composite revealed a shell-core system with a sustained release of calcium ions.
ISSN:2042-6496
2042-650X
DOI:10.1039/d1fo02134f