Effects of type II collagen hydrolysates on osteoarthritis through the NF-κB, Wnt/β-catenin and MAPK pathways

Osteoarthritis (OA), a degenerative disease, has attracted extensive attention all over the world. In this study, a rat model involving medial meniscus resection (MMx) and anterior to medial collateral ligament (ACL) operation was successfully established to study the effects of bovine cartilage hyd...

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Bibliographic Details
Published in:Food & function 2022-02, Vol.13 (3), p.1192-125
Main Authors: Hao, Li, Ma, Chengcheng, Li, Zhaoxia, Wang, Yanchao, Zhao, Xue, Yu, Mingxiao, Hou, Hu
Format: Article
Language:English
Subjects:
Animals
Arthritis
beta Catenin - metabolism
Biomedical materials
Cartilage
Cartilage diseases
Cbfa-1 protein
Cells, Cultured
Collagen
Collagen (type II)
Collagen Type II - metabolism
Degeneration
Disease Models, Animal
Female
Functional foods & nutraceuticals
Hydrolysates
Interleukin 6
Knee
MAP kinase
Meniscus
Mitogen-Activated Protein Kinase Kinases - metabolism
mRNA
NF-kappa B - metabolism
NF-κB protein
Oral administration
Osteoarthritis
Osteoarthritis - metabolism
Peptides
Proteomics
Rats
Rats, Sprague-Dawley
Wnt protein
Wnt Signaling Pathway
β-Catenin
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Summary:Osteoarthritis (OA), a degenerative disease, has attracted extensive attention all over the world. In this study, a rat model involving medial meniscus resection (MMx) and anterior to medial collateral ligament (ACL) operation was successfully established to study the effects of bovine cartilage hydrolysates rich in type II collagen peptides (BIIP) on cartilage protection. The results of histological analysis indicated that oral administration of BIIP at doses of 200 and 500 mg kg −1 d −1 ameliorated cartilage degeneration. Moreover, the potential targets of BIIP affecting OA in vivo were studied by proteomics, and the effects of BIIP on OA through signaling pathways, such as NF-κB, Wnt/β-catenin and MAPK, were further explored at mRNA and protein levels. BIIP downregulated the expression of IL-6, RUNX2, NF-κB p65, HIF-2α, β-catenin and p-JNK, which may be the main factor leading to the prevention of OA. These results suggest that BIIP can be used as a novel potential substance of functional foods to exert chondroprotective action. Type II collagen peptides could significantly influence OA.
ISSN:2042-6496
2042-650X
DOI:10.1039/d1fo03414f