Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme

A Ru-containing single chain nanoparticle (SCNP) was prepared in three steps using radical polymerization of pentafluorophenylacrylate, post-polymerization functionalization with three different alkylamines, and coordination of Ru. The polymer was characterized by 1 H NMR, 19 F NMR, UV-vis, and DLS....

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Bibliographic Details
Published in:Polymer chemistry 2021-11, Vol.12 (46), p.6755-676
Main Authors: Garcia, Edzna S, Xiong, Thao M, Lifschitz, Abygail, Zimmerman, Steven C
Format: Article
Language:English
Subjects:
Alkylamines
Catalysis
Catalytic activity
Fluorescence
Galactosidase
Nanoparticles
NMR
Nuclear magnetic resonance
Polymer chemistry
Polymerization
Ruthenium
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Summary:A Ru-containing single chain nanoparticle (SCNP) was prepared in three steps using radical polymerization of pentafluorophenylacrylate, post-polymerization functionalization with three different alkylamines, and coordination of Ru. The polymer was characterized by 1 H NMR, 19 F NMR, UV-vis, and DLS. The catalytic activity of the Ru-SCNP for Ru-catalyzed cleavage of allylcarbamates was evaluated by fluorescence spectroscopy and a higher percent conversion and initial rate of reaction was observed when compared to that of the free catalyst in buffer and cell media. The catalytic SCNP was also shown to perform tandem catalysis with β-galactosidase. A Ru-containing single-chain nanoparticle (SCNP) performs allylcarbamate cleavage reactions in biologically relevant environments more efficiently than free catalyst and works in synergy with a natural enzyme to perform tandem catalysis.
ISSN:1759-9954
1759-9962
DOI:10.1039/d1py01255j