Engineered flavoproteins as bioorthogonal photo-triggers for the activation of metal-based anticancer prodrugs

A multifunctional hybrid constructed for controlling the delivery and activation of Pt anticancer agents in vitro is described herein. We employed consensus tetratricopeptide repeat protein (CTPR) for the covalent co-anchoring of riboflavin (photocatalyst) and a Pt( iv ) prodrug complex. The Pt-load...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2023-04, Vol.59 (32), p.4754-4757
Main Authors: Mazzei, Laura F, Gurruchaga-Pereda, Juan, Martínez, Álvaro, Martínez, Javier Calvo, Salassa, Luca, Cortajarena, Aitziber L
Format: Article
Language:English
Subjects:
Anticancer properties
Antineoplastic Agents - pharmacology
Cell Line, Tumor
Cisplatin
Flavoproteins
Photocatalysis
Prodrugs - pharmacology
Riboflavin
Riboflavin - pharmacology
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Summary:A multifunctional hybrid constructed for controlling the delivery and activation of Pt anticancer agents in vitro is described herein. We employed consensus tetratricopeptide repeat protein (CTPR) for the covalent co-anchoring of riboflavin (photocatalyst) and a Pt( iv ) prodrug complex. The Pt-loaded flavoprotein induced a 40% reduction in PANC-1 cell viability as a result of the photocatalytic formation of cisplatin. Riboflavin-functionalized repeat proteins enable controlling the photocatalytic activation and delivery of Pt anticancer agents in vitro .
ISSN:1359-7345
1364-548X
DOI:10.1039/d2cc06363h