SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids

Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface...

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Published in:Chemical communications (Cambridge, England) England), 2023-03, Vol.59 (22), p.3249-3252
Main Authors: Skinner, William H, Robinson, Nicola, Hardisty, Gareth R, Fleming, Holly, Geddis, Ailsa, Bradley, Mark, Gray, Robert D, Campbell, Colin J
Format: Article
Language:English
Subjects:
Cellular structure
Extracellular Matrix
Gold - chemistry
Humans
Hydrogen-Ion Concentration
Metal Nanoparticles - chemistry
Nanoparticles
Organoids - metabolism
Raman spectra
Self-assembly
Spectrum Analysis, Raman
Stem Cells
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Summary:Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM. SERS microsensors (SERS-MS) are polymer microparticles coated in gold nanoparticles and functionalised with mercaptobenzoic acid. SERS-MS were incorporated into human airway organoids to measured pH optically.
ISSN:1359-7345
1364-548X
DOI:10.1039/d2cc06582g