NaBiF 4 upconversion nanoparticle-based electrochemiluminescent biosensor for E. coli O157 : H7 detection

• Published in: RSC advances 2022-10, Vol.12 (46), p.30174-30180
• Main Authors: Liu, Danqing, Lv, Xingxing, Zhao, Chaoyue, Li, Jiayue, Huang, Jinmei, Weng, Ling, He, Liangcan, Liu, Shaoqin
• Format: Article
• Language: English
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/D2RA05217B

Foodborne or water-borne pathogens pose great threats to human beings and animals. There is an urgent need to detect pathogens with cheap, rapid and sensitive point-of-care diagnostic assays. Herein, we report the electrochemiluminescent (ECL) behaviors of NaBiF  : Yb /Er upconversion nanoparticles (UCNPs) which were synthesized a fast and environment-friendly method at room temperature for the first time. The UCNPs together with K S O exhibit high ECL intensity and stable cathodic signals. Further, the Au nanoparticles (Au NPs) and Anti- O157 : H7 antibody were assembled on the surface of UCNPs successively to construct a novel ECL immunosensor for the detection of deadly O157 : H7. The as-prepared ECL immunosensor reveals high sensitivity to O157 : H7 in a linear range of 200-100 000 CFU mL , and the minimum detection limit could reach up to 138 CFU mL . The designed UCNP-based biosensor demonstrates high specificity, good stability and remarkable repeatability, and the strategy will provide a sensitive and selective method for rapid detection of O157 : H7 in food safety and preclinical diagnosis.